栽培大豆品种间RAPD标记的多态性分析及聚类分析

应用从６０００多份大豆种质资源中筛选的２１个抗大豆花叶病毒病的品种或品系及７个感病的品种或品素，选用２０个随机引物，对总ＤＮＡ进行了随机扩增。有１８个引物扩增得得到了稳定的ＲＡＰＤ图谱。ＯＰＨ－０６和ＯＰＨ－１０未能扩增到ＲＡＰＤ产物。

POLYMORPHISMS AND CLUSTER ANALYSIS OF CULTIVATED SOYBEANS WITH RAPD

In present study, twenty-one soybean accessions with host resistance to different SMV strains and 7 susceptible cultivars or lines were analysed for their genetic diversity using RAPD. Twenty 10-mer random primers were used to amplify the total DNA from 28 soybean accessions. The reults showed that repriducible amplified products could be got with 18 primers. The length of amplification products are between 0.6-4.6kb. Totally 165 RAPD bands were identified, of which, 104 bands were commom in all the accessions and 61 bands were polymorphic between any two accessions. The polymorphic bands accounted for 37% of the total amplified bands.These primers could be divided into six groups.1) no amplification products; 2) no polymorphisms between amplification products; 3) polymorphic bands accounted for less than 15% of the total bands; 4) polymorphisms between 15%-30%; 5) polymorphisms between 30%-50%; 6) polymophisms above 50%. Based on the genetic distance matrix, cluster analysis were conducted. The results showed that these soybean accessions could be divided into five groups. Cultivars and lines clustered together in any group or subgroup were consistent with their pedigree and geographical distribution.