不同介质对真姬菇菌种保藏效果的对比

选择适宜的菌种保藏介质,是有效保障真姬菇菌种品质的重要一环,不仅关系到真姬菇栽培者的直接利益,也关系到种质资源的保存与延续。通过测定菌丝生长速率和菌丝脱氢酶活力,对22种真姬菇菌种保藏介质的保藏效果进行了对比分析。结果表明0.1%的PEG6000溶液对真姬菇菌种保藏6个月和9个月的整体保藏效果最好,其次是0.1%的PEG10000溶液与超纯水,再次是0.8%葡萄糖溶液和矿泉水。0.6%葡萄糖溶液仅适于真姬菇菌种9个月的保藏,纯净水仅适于真姬菇菌种6个月的保藏。其他介质的保藏效果略差。

Effect Comparison of Different Preservation Media for Strains of Hypsizygus marmoreus

Selection of suitable preservation media is an important part of effective preservation of Hypsizygus marmoreus strain.It is not only concerns the direct benefit of H. marmoreus cultivators, but also concerns the preservation and continuation of germplasm resources. In this paper, the preservation effects of 22 preservation media of H. marmoreus strain were compared and analyzed by measuring mycelial growth rate and mycelial dehydrogenase activity. The results showed that 0.1% PEG6000 solution had the best effect for the preservation of 6 months and 9 months, followed by 0.1% PEG10000 solution and ultrapure water, and 0.8% glucose solution and mineral water were the third. 0.6% glucose solution was only suitable for 9 months’ preservation of H. marmoreus strain, and pure water was only suitable for 6 months’ preservation. The preservation effects of other media were slightly poor.