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| 树舌液体发酵的培养基优化 | |
| 引用本文： | 黄达明,李欣,黄翀,张志才,孙文敬.树舌液体发酵的培养基优化[J].中国食用菌,2011,30(3):28-31. |
| 作者姓名： | 黄达明 李欣 黄翀 张志才 孙文敬 |
| 作者单位： | 1. 江苏大学食品与生物工程学院,江苏,镇江,212013;江西省生物发酵食品添加剂工程技术研究中心,江西,上饶,334221 2. 江苏大学食品与生物工程学院,江苏,镇江,212013 3. 南京邮电大学通信与信息工程学院,江苏,南京,210046 4. 江苏大学食品与生物工程学院,江苏,镇江,212013;北京绿科天成生物有限公司,北京,100089 |
| 基金项目： | 第44批中国博士后基金，南通市社会发展基金，江西省科技条件平台建设项目，江苏高校优势学科建设工程资助项目 |
| 摘 要： | 以树舌发酵液提高离体小鼠肝组织的糖耗量为指标,通过单因素试验研究不同碳源、氮源、生长因子、pH以及无机盐对树舌发酵液生长的影响,筛选出适合的碳源、氮源及无机盐,并进一步通过正交试验确定各组分之间的相互作用。试验结果表明,树舌发酵液生长的最适培养基组成为葡萄糖5 g.L-1、甘露醇5 g.L-1、蛋白胨1 g.L-1、硫酸铵1g.L-1、硫酸亚铁0.5 g.L-1、氯化锌0.5 g.L-1、氯化钾0.5 g.L-1。树舌发酵液在28℃、150 r.min-1发酵培养5 d,发酵液糖耗量可高达到88.62%,明显高于未优化的培养基。 |
| 关 键 词： | 树舌 培养基优化 葡萄糖糖耗量 正交试验 |
Optimizing Components of Ganoderma applanatum's Medium by Liquid Cultured |
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| HUANG Da-ming,LI Xin,HUANG Chong,ZHANG Zhi-cai,SUN Wen-jing.Optimizing Components of Ganoderma applanatum's Medium by Liquid Cultured[J].Edible Fungi of China,2011,30(3):28-31. | |
| Authors: | HUANG Da-ming LI Xin HUANG Chong ZHANG Zhi-cai SUN Wen-jing |
| Institution: | HUANG Da-ming1,2,LI Xin1,HUANG Chong3,ZHANG Zhi-cai1,4,SUN Wen-jing1,2(1.School of Food and Biological Engineering,Jiangsu University,Zhenjiang Jiangsu 212013,2.Engineering Research Center for Food Additive of Biological Fermentation,Shangrao Jiangxi 334221,3.College of Telecommunications and Information Engineering,Nanjing University of Posts and Telecommunications,Nanjing Jiangsu 210046,4.Beijing Green Technology and Natural Biotechnology Co.Ltd.,Beijing 100089) |
| Abstract: | The fermentation condition of Ganoderma applanatum were studied with glucose consumption of isolated liver as an index.In this paper,fermentation of glucose consumption selected by single factor for carbon sources,nitrogen sources,growth factor,inorganic salts and pH.Their interactions were decided by orthogonal test.Its optimized culture medium ingredients were glucose 5 g·L-1,mannitol 5 g·L-1,peptone 1 g·L-1,ammonium sulfate 1 g·L-1,ZnCl2 5 g·L-1,FeSO4 0.5 g·L-1,KCl 0.5 g·L-1.Glucose consumption was achieved 88.62% under the condition of 150 r·min-1 for 5 d at 28℃ through the fermentation of the optimized culture,which was significantly higher than basal medium. |
| Keywords: | Ganoderma applanatum Glucose consumption Medium optimization Orthogonal design |
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