| 松口蘑菌丝分离及其RAPD—PCR鉴定 |
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| 引用本文： | 刁桂萍,孟雪,邹莉.松口蘑菌丝分离及其RAPD—PCR鉴定[J].中国食用菌,2011,30(2):46-48. |
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| 作者姓名： | 刁桂萍 孟雪 邹莉 |
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| 作者单位： | 东北林业大学,黑龙江,哈尔滨,150040 |
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| 基金项目： | 哈尔滨市科技攻关项目，中央高校基本科研业务费专项资金 |
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| 摘 要： | 采用组织分离法，对东宁县的松口蘑进行了分离培养。通过显微观察及RAPD—PCR技术对松口蘑子实体及其相应的培养物之间进行了亲缘关系鉴定。结果表明，菌褶为松口蘑组织分离的最适宜部位，其最佳分离培养基为冈叮，即PDA＋黑木耳二级种浸出液＋麦麸。RAPD—PCR结果显示，松口蘑子实体与分离物之间的DNA指纹相似系数在0．97～1．00之间，表明松口蘑子实体与其相应的培养物在种质上是一致的。
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| 关 键 词： | 松口蘑 组织分离 RAPD—PCR |
Isolation of Tricholoma matsutake and It's RAPD-PCR Identification |
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| DIAO Gui-ping,MENG Xue,ZOU Li.Isolation of Tricholoma matsutake and It's RAPD-PCR Identification[J].Edible Fungi of China,2011,30(2):46-48. |
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| Authors: | DIAO Gui-ping MENG Xue ZOU Li |
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| Institution: | DIAO Gui-ping,MENG Xue,ZOU Li(Northeast Forestry University,Haerbin Heilongjiang 150040) |
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| Abstract: | In this study, the mycelia of Tricholoma matsutake from Dongning were cultivated by the method of tissue separation and identified using RAPD-PCR. The results showed that lamellae were suitable for the tissue separation and well induced on PWJ medium. By RAPD-PCR analysis, DNA fingerprint similarity coefficient was 0.97-1.00, that implied the mycelia were in- deed from their corresponding fruiting body. |
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| Keywords: | Tricholoma matsutake Tissue separation RAPD-PCR |
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