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| 几种测定灰树花多糖中蛋白质含量方法的比较研究 | |
| 引用本文： | 王卫国,吴强,胡宝坤,包东武,赵永亮.几种测定灰树花多糖中蛋白质含量方法的比较研究[J].中国食用菌,2003,22(1):27-30. |
| 作者姓名： | 王卫国 吴强 胡宝坤 包东武 赵永亮 |
| 作者单位： | 1. 南阳理工学院生物与化学工程系 2. 南阳理工学院医学院微生物室,河南,南阳,473004 3. 四川农业大学农学院,四川,雅安,6250014 |
| 基金项目： | 河南省南阳市科委资助的攻关项目，项目编号为No . 0 0 0 30 8.TEL :0 377- 312 0 387(H)，312 16 0 5 (0 )FAX :0 377- 312 14 0 4 |
| 摘 要： | 用双缩脲法、紫外吸收法、考马斯亮蓝G－250染色法三种方法对灰树花多糖中蛋白质含量进行测定，并同微量凯氏定氮法进行对比。结果表明：在蛋白质量浓度0．1-1.0mg/mL范围内，其测量误差分别为双缩脲法大于50％，紫外吸收法大于30％，考马斯亮蓝G－250染色法最低可小于3％，考马斯亮蓝G－250染色法简单，迅速，且相对较为准确，是测定灰树花多糖中蛋白质含量的有效方法。 |
| 关 键 词： | 灰树花多糖 蛋白质含量 测定方法 考马斯亮蓝G－250染色法 |
| 文章编号： | 1003-8310(2003)01-0027-04 |
| 修稿时间： | 2002年6月13日 |
Comparative Study on Determination Methods of Residual Protained in Grifolan |
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| WANG Wei-guo,WU Qiang,HU Bao-kun,BAO Dong-wu,ZHAO Yong-liang .Biochemical Engineering Dept.,Nanyang Institute of Technology,Nanyang,Henan ,PRC..Medical School,Nanyang Institute of Technology,Nanyang,Henan ,PRC..Agricultural Dept.,Sichuan Agricultural University.Ya'an,Sichuan,PRC..Comparative Study on Determination Methods of Residual Protained in Grifolan[J].Edible Fungi of China,2003,22(1):27-30. | |
| Authors: | WANG Wei-guo WU Qiang HU Bao-kun BAO Dong-wu ZHAO Yong-liang Biochemical Engineering Dept Nanyang Institute of Technology Nanyang Henan PRCMedical School Nanyang Institute of Technology Nanyang Henan PRCAgricultural Dept Sichuan Agricultural UniversityYa'an Sichuan PRC |
| Institution: | WANG Wei-guo,WU Qiang,HU Bao-kun,BAO Dong-wu,ZHAO Yong-liang 1.Biochemical Engineering Dept.,Nanyang Institute of Technology,Nanyang,Henan 473004,PRC.2.Medical School,Nanyang Institute of Technology,Nanyang,Henan 473002,PRC.3.Agricultural Dept.,Sichuan Agricultural University.Ya'an,Sichuan,625014,PRC. |
| Abstract: | The Determination methods of residual proteins contained in grifolan such as Biuret Reaction,UV absorption and the dying method with Coomassie Brilliant Fluka G-250 were studied and compared with the Kjeldahl's minor nitrogendetermining method.The results indicated that the determination deviations are more than 50% for Biuret Reaction,more than 30% for UV absorption method,and less than 3% for the dying method with Coomassie Brilliant Fluka G-250 at least while the concentration of proteins ranges from 0 1 to 1 0 mg/ml.The dying method with Coomassie Brilliant Fluka G-250 possesses the properties of simplicity,rapidness and exactness,so it's an effective method of determining the content of proteins contained in grifolan. |
| Keywords: | Grifolan Content of protenins Determination methods Dying method with Coomasie Brilliant Fluka G-250 |
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