灵芝孢子油检测标准与其抗肿瘤活性的关系

利用多种分析检测手段和肿瘤细胞实验,研究了灵芝孢子油的质量检测参数与其抗肿瘤细胞活性的关系,为灵芝孢子油质量检测标准的建立提供实验参考依据。取6种不同的经CO2超临界提取的灵芝孢子油样品,用KOH标准滴定法测酸价、KI-I2法检测过氧化值、紫外-可见光分光光度法测总三萜。同时利用肿瘤细胞检测6种样品对人恶性乳腺癌细胞（MT-1）和淋巴瘤细胞（Jurka）t的抑制作用。结果表明,样品的酸价与总三萜含量和其抗肿瘤效果呈正相关,6种样品的过氧化值变化不大。高酸价样品1,其总三萜含量（16.00%）较高,在1μL·mL-1浓度抑制时,能够抑制绝大部分肿瘤细胞的生长,具有较高的抗肿瘤活性。

The Relationship of Ganoderma lucidum Spore Oil Quality Testing Indicators and Its Anti-tumor Cell Activity

The relationship of Ganoderma lucidum spore oil quality testing standards and its anti-tumor cell activity to provide parameters for the establishment of testing standards were studied. Ganoderma lucidum spore oil was extracted by CO2 supercritical fluid, which had six different samples. The acid value, the peroxide value, total triterpenoids were determined. The inhibition effect of Ganoderma lucidum spore oil on human malignant breast cancer cells （MT-1） and human leukemia cells （Ju-rkat） were studied by cell culture method. The results showed that anti-tumor effect was positively correlated with acid value and the total level of triterpenoids.