| 甘菊水培体系的建立 |
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| 引用本文： | 牛雅静,黄河,杨可,王斌,戴思兰.甘菊水培体系的建立[J].中国园艺文摘,2011,27(11):1-3,26. |
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| 作者姓名： | 牛雅静 黄河 杨可 王斌 戴思兰 |
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| 作者单位： | 北京林业大学园林学院,北京,100083 |
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| 基金项目： | 国家自然科学基金项目(31071823):光照调节菊花花青素合成的分子机理，国家自然科学基金项目(30871726):光周期诱导菊花成花的分子机理研究 |
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| 摘 要： | 为探索甘菊Chrysanthemum lavandulifolium(Fisch. ex Trautv.)Makino]在非生物胁迫下以及营养物质代谢中精准的生理、基因表达变化机理,研究通过筛选外植体的种类和营养液的浓度,建立了甘菊水培体系。结果表明:水培中,播种苗的成活率和生根率极显著高于1年生大苗和茎段插条;在长时间的水培中,使用标准Hoagland和1.5倍Hoagland培养液,其畸形叶率极显著低于1/2和1/4Hoagland培养液,并且株高极显著高于另外2个浓度。结合科研需求,认为组培播种苗、标准Hoagland营养液是甘菊水培的最佳外植体和培养条件。
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| 关 键 词： | 甘菊 水培 外植体 营养液 |
Establishment of Hydroponics Culture System for Chrysanthemum lavadulifolium |
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| Authors: | NIU Ya-jing HUANG He YANG Ke WANG Bin DAI Si-lan |
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| Institution: | NIU Ya-jing HUANG He YANG Ke WANG Bin DAI Si-lan |
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| Abstract: | In order to explore the accurate mechanism of physiology and gene expression under abiotic stress and nutrient metabolism for Chrysanthemum lavadulifolium,we screened the explants and concentration of nutrient solution,and established the hydroponics culture system.The results indicate that in hydroponics culture,the survival rate and rooting rate are significantly higher in seedings rather than yearlings or cuttings;for a long time culture,the abnormal leaf rate is lower and the height is higher in normal and 1.5 times Hoagland solution than 1/2 or 1/4 Hoagland solution.Considering the actual demand in research,we choose seedings sown in MS medium,normal Hoagland solution as the best explants and culture medium. |
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| Keywords: | Chrysanthemum lavadulifolium hydroponics culture explants nutrient solution |
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