掌裂草葡萄对NaCl胁迫的生理适应性研究

掌裂草葡萄是观赏价值很高的多年生藤本植物，在寒地园林垂直绿化中具有很好的推广价值。目前对其抗盐性研究未见报道。本试验通过对掌裂草葡萄一年生播种苗进行不同浓度的NaCl胁迫处理，研究其细胞膜透性，渗透调节物质（可溶性糖、可溶性蛋白）、保护酶活性（SOD、POD）在盐胁迫下的变化规律，揭示掌裂草葡萄的耐盐性。结果表明：掌裂草葡萄对NaCl胁迫有一定的应激性，胁迫使可溶性蛋白含量和SOD活性迅速增大；各处理随胁迫时间的延长膜透性逐渐增大，胁迫浓度越大，电解质外渗越多。400mmol／L胁迫处理第18d，电解质外渗达50％以上，处理第30d植株死亡；浓度300mmol／L及以上时，可溶性糖、可溶性蛋白和SOD活性在处理第18d开始迅速下降，POD活性在处理第30d下降，可溶性糖含量和SOD活性分别在第24d和第30d低于对照。由此推断，300mmol／L及以上的NaCI胁迫不利于掌裂草葡萄的生长。

Physiological Adaptability of Aconiteleaf Ampelopsis to NaCl Stress

AconiteleafAmpelopsis is an kind of excellent garden virescence plant whose leaves,flowers and fruit all have a good view and admire value.But,recently, there is no report of research to its resistance.This research selected one year old seedlings of Aconiteleaf Ampelopsis,treated under NaCl stress with different salinity.By analyzing changes of electrolyte leakage rate,soluble sugar, soluble protein,SOD and POD in leaves,its NaCl tolerance is found out.The results indicate that,AconiteleafAmpelopsis had definite irritability to NaCl stress,the stress made soluble protein content and SOD activity rapidly augmented.Membrane permeability gradually increased over time,and electrolyte exuded more and more along with the stress enhanced.Under the 400mmol/L NaCl treated at the 18th day, electrolyte leakage rate exceeded 50%,and the plant died at the 30th day.When salinity over 300mmol/L,soluble sugar, soluble protein and SOD activity rapidly declined at the 18th day, POD activity declined at the 30th day, soluble sugar content and SOD activity lower than contrast respectively at the 24th day and the 30th day.lt was concluded that,300mmol/L and higher salinity was not propitious for Aconiteleaf Ampelopsis to grow.