| 小花鬼子针组织培养及无性系建立的研究 |
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| 引用本文： | 黄葳,施玉婷,杨微,宁淑香,张冬艳.小花鬼子针组织培养及无性系建立的研究[J].中国园艺文摘,2010(7):9-12. |
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| 作者姓名： | 黄葳 施玉婷 杨微 宁淑香 张冬艳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 基金项目： | 辽宁省高等教育教学改革研究项目，辽宁师范大学教学改革项目 |
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| 摘 要： | 以生长旺盛的小花鬼子针嫩茎的腋茅为材料,进行愈伤组织诱导,愈伤组织和不定茅分化,试管苗生根、移栽、扦插和移植研究,建立起小花鬼子针的无性系。结果证明:MS+BA0.3mg/L+2,4-D1.2～1.6mg/L是诱导嫩茎形成具有分化能力愈伤组织的理想培养基;1/2MS+AgNO_31.5mg/L+BA0.3mg/L+NAA0.2mg/L是诱导愈伤组织和不定茅分化的理想培养基;1/3MS+NAA0.1mg/L+IAA0.3mg/L是小花鬼子针试管苗生根培养和生根继代培养的理想培养基。炉灰渣是试管苗移栽和扦插的理想基质。试管苗移植后长势整齐、旺盛,各种生物学性状均与野生植株一致。
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| 关 键 词： | 小花鬼子针 组织培养 无性系 |
Tissue Culture and Establishment of Bidens parviflora Clone |
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| Authors: | HUANG Wei SHI Yu-Ting YANG Wei NING Shu-Xiang ZHANG Dong-Yan |
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| Institution: | HUANG Wei SHI Yu-Ting YANG Wei NING Shu-Xiang ZHANG Dong-Yan |
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| Abstract: | Growth, differentiation, rooting and transplanting of axillary bud were studied and establishment of Bidens parviflora clone was also conducted. The results indicate that the ideal medium for callus induction is MS＋BA0.3mg/ L＋2,4-D1.2-1.6 mg/L. The best medium for differentiation is 1/2MS＋AgNO31.5mg/L＋BA0.3mg/L＋NAA0.2mg/L. The medium of 1/3MS＋ NAA0.1mg/L＋IAA0.3mg/L is suitable for rooting. The cinder is the ideal transplanting matrix. The tube seeding of Bidens parviflora are transferred into the parterre and keep the variant characters. |
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| Keywords: | Bidens parviflora tissue culture clone |
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