铁炮百合叶片的组织培养研究

以铁炮百合的叶片为外植体进行离体培养研究，结果表明：一定浓度范围内的6-BA、KT、2，4-D和NAA间的配组，能诱导不定茅形成。当激素组合为0．5mg／LNAA＋1．0mg／LKT＋0．3mg／L6-BA＋1．0mg／L2，4-D时，铁炮百合的愈伤组织诱导率、出茅率与平均出茅数均达到最高。最佳继代增殖培养基为MS，小苗在1／2MS＋0．1mg／L6-BA上容易生根。

Study on Tissue Culture of Lilium longiflorum Thumb

In this paper, the effects of different hormone combinations designed for lily＇s leaves on callus and differentiation were studied. The results showed that a certain range of concentration 6-BA,KT and 2,4-D combined with NAA were more profit for callus induction and differentiation, and the best effect of hormone combination for Lilium longiflorum Thumb. on callus inducing and bud inducting was 0.5mg/L NAA＋1.0mg/L KT＋0.3mg/L 6-BA＋1.0mg/L 2,4-D.The optimum subculture medium was MS, the best medium for rootage of rootless bulbs and seedlings was 1/2MS＋0.1 mg/L 6-BA.