蔗糖浓度对葡萄试管苗生长及光合作用的影响

糖为植物生长发育提供能量,也是形态建成的物质基础。但培养基的蔗糖浓度对葡萄试管苗生长及光合作用的影响研究较少,以‘黑比诺’葡萄试管苗为试材,以GS为基本培养基、蔗糖浓度30g/L为对照,分别研究蔗糖浓度0、60g/L对‘黑比诺’葡萄试管苗生理指标和光合参数的影响。结果表明:蔗糖浓度为30g/L试管苗生长状况最佳,试管苗干鲜重、叶绿素含量、可溶性糖含量均显著高于其他2个处理,总糖含量无显著差异;蔗糖浓度60 g/L处理试管苗叶片可溶性蛋白含量显著高于其他2个处理,在高糖及无糖处理下,试管苗叶片蒸腾速率(E)、气孔导度(Gs)与净光合速率(Pn)均显著低于正常浓度处理;叶绿素a、叶绿素b、光合电子传递速率(ETR)、最大量子产量(Fv/Fm)在蔗糖浓度30 g/L处理下均显著高于其他2个处理,叶片中不同激素受蔗糖浓度的影响各不相同。综上,蔗糖浓度能够影响葡萄试管苗生物量、叶绿素相对含量、可溶性蛋白、可溶性糖及总糖含量变化,培养基蔗糖浓度过高或过低会影响叶绿素荧光参数的变化,进而影响光合作用。

Effects of sucrose concentration on growth and photosynthesis of grape plantlets in vitro

Sugar is an important material for plant,which can provide energy for plant growth and development.However,there are few studies on how the different concentrations of sucrose affect the growth and photosynthesis of grape plantlets in vitro.Therefore,the grape‘Pinot Noir’plantlet was used as the test material and GS as basic medium.The sucrose concentration was 0 g/L,30 g/L,60 g/L respectively.The effects of different sucrose concentration on physiological indexes and photosynthetic parameters of grape‘Pinot Noir’plantlet were studied the growth condition of the plantlet was the best when the sucrose concentration was 30 g/L.The dry weight,chlorophyll content,and soluble sugar content of plantlet were significantly higher than the other two treatments but there was no significant difference in the total sucrose content.Under GS0 and GS2 treatments,the transpiration rate(E),stomatal conductance(Gs)and photosynthetic rate(Pn)of plantlet significantly lower than GS.Chlorophyll a,chlorophyll b,ETR,Fv/Fm under 30 g/L sucrose treatment were significantly higher than those of the other two treatments.Different hormones in plantlet leaves were affected differently by sucrose concentration.The concentration of sucrose can affect the biomass of plantlet,the relative content of chlorophyll,soluble protein,soluble sugar and total sugar.Higher or lower sucrose concentration in the medium will affect the chlorophyll fluorescence parameters,and thus affect photosynthesis.