| 茭白黑粉菌脉冲电泳分析方法 |
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| 引用本文： | 王晓清,张敬泽,褚福强,等.茭白黑粉菌脉冲电泳分析方法[J].长江蔬菜,2011(16):82-84. |
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| 作者姓名： | 王晓清 张敬泽 褚福强 等 |
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| 作者单位： | 浙江大学农业与生物技术学院,杭州,310058 |
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| 基金项目： | 公益性行业(农业)科研专项经费项目“水生蔬菜产业技术体系研究与示范”(200903017-03) |
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| 摘 要： | 采用脉冲电泳法对茭白黑粉菌染色体进行核型分析。分析结果表明,茭白黑粉菌脉冲电泳样品处理方法包括原生质体的制备、电泳参数的设置等,对试验结果有一定影响,在制备电泳样品时,包埋的原生质体或孢子的浓度不能太低,蛋白酶K的浓度不能过高,低熔点琼脂糖的浓度不能太低,此外还应确保电泳槽水平,并且电泳结束后及时取出凝胶。
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| 关 键 词： | 茭白黑粉菌 原生质体 脉冲电泳 核型分析 |
Protocol for pulsed field gel electrophoresis of Ustilago esculenta |
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| WANG Xiaoqing,ZHANG Jingze,CHU Fuqiang,ZHANG Yanli,GUO Deping.Protocol for pulsed field gel electrophoresis of Ustilago esculenta[J].Journal of Changjiang Vegetables,2011(16):82-84. |
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| Authors: | WANG Xiaoqing ZHANG Jingze CHU Fuqiang ZHANG Yanli GUO Deping |
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| Institution: | WANG Xiaoqing,ZHANG Jingze,CHU Fuqiang,ZHANG Yanli,GUO Deping(College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058) |
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| Abstract: | Karyotype of Ustilago esculenta was analyzed by pulsed field gel electrophoresis.The experiment results were affected by the sample treatment methods of pulsed field gel electrophoresis of U.esculenta,including protoplast preparation and entrapment.While preparing the test samples,the concentration of embedded protoplast or spore and low melting point agarose can't be too low,and the concentration of proteinase K can't be too high.In addition,the electrophoresis tank should be placed horizontally,and the ge... |
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| Keywords: | Ustilago esculenta Protoplast Entrapment Pulsed field gel electrophoresis Karyotype |
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