| 马铃薯总RNA提取和鉴定方法的改进 |
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| 引用本文： | 白云凤,郭志华,白冬梅,王小琦,张维锋.马铃薯总RNA提取和鉴定方法的改进[J].园艺学报,2007,34(4):1059-1062. |
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| 作者姓名： | 白云凤 郭志华 白冬梅 王小琦 张维锋 |
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| 作者单位： | (山西省农业科学院作物遗传研究所, 太原030031) |
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| 基金项目： | 国家自然科学基金;山西省留学回国人员科研项目;山西省农业科学院博士基金 |
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| 摘 要： | 对植物总RNA的提取和鉴定方法作了改进。以马铃薯叶片和茎为材料,分别用常规苯酚法和改进法提取总RNA,通过甲醛变性胶和非变性胶及紫外光谱分析,表明用改进法提取的总RNA与常规苯酚法无明显差异,RNA完整性良好,可以用普通琼脂糖凝胶代替甲醛变性胶电泳分析RNA的完整性。RT-PCR结果表明,以改进法提取的总RNA可以用于分子克隆和基因表达分析等分子生物学实验。利用改进法提取植物总RNA,能降低成本,节约时间,也避免了使用DEPC和甲醛等试剂。
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| 关 键 词： | 马铃薯 总RNA提取 |
| 文章编号： | 0513-353X（2007）04-1059-04 |
| 收稿时间： | 2007-01-15 |
| 修稿时间： | 2007-01-15 |
An Improved Method for Extraction and Identification of Potato RNAs |
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| BAI Yun-feng,GUO Zhi-hua,BAI Dong-mei,WANG Xiao-qi,ZHANG Wei-feng.An Improved Method for Extraction and Identification of Potato RNAs[J].Acta Horticulturae Sinica,2007,34(4):1059-1062. |
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| Authors: | BAI Yun-feng GUO Zhi-hua BAI Dong-mei WANG Xiao-qi ZHANG Wei-feng |
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| Institution: | (Crop Genetics Institute, Shanx i Academ y of Agricultural Sciences, Taiyuan 030031,China) |
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| Abstract: | An improved method of extracting and identifying total RNAs from potato leaf and stem was put forward. The electrophoretic patterns and absorbance at 260 nm and 280 nm in a UV-Vis spectrophotometer revealed that the total RNAs of potato extracted by this method has good completeness and high purity. Furthermore, the result of RT-PCR indicated that the RNAs prepared with this improved method could meet the needs of most molecular biological experiment including gene cloning and expression analysis. |
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| Keywords: | Potato Total RNAs extraction |
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