| 袋鼠花的组织培养 |
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| 引用本文： | 刘春,穆鼎.袋鼠花的组织培养[J].园艺学报,2003,30(1):113-115. |
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| 作者姓名： | 刘春 穆鼎 |
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| 作者单位： | (中国农业科学院蔬菜花卉研究所, 北京100081) |
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| 摘 要： | 对袋鼠花( Anigozanthos) 组织培养研究表明: 外植体消毒以0. 1 %升汞溶液浸泡, 7～8 min 为宜; 不同浓度激素对其愈伤组织形成、分化以及根的形成有不同的影响, 随着62BA 浓度的增加, 愈伤组织的诱导率有增加的趋势; 生长素与分裂素的比例决定着愈伤组织的分化; 小苗在1/ 2 MS 培养基上生根最好, 6-BA 对其生根有抑制作用。各培养阶段最适培养基: (1) 愈伤, MS + 6-BA 1 mg/L + NAA 0. 1 mg/L +3 %活性炭; (2) 芽诱导, MS + 6-BA 0. 5 mg/L + NAA 0. 5 mg/L ; (3) 生根, 1/ 2 MS + IBA 0. 2 mg/L。
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| 关 键 词： | 袋鼠花 组织培养 |
| 文章编号： | 0513-353X(2003)01-0113-02 |
| 收稿时间： | 2002-1-24 |
| 修稿时间： | 2002年1月24日 |
The Studies of the Tissue Culture of Anigozanthos(Kangaroo paws) |
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| Liu Chun and Mu Ding.The Studies of the Tissue Culture of Anigozanthos(Kangaroo paws)[J].Acta Horticulturae Sinica,2003,30(1):113-115. |
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| Authors: | Liu Chun and Mu Ding |
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| Institution: | 1. Department of Thoracic and Cardiac Surgery,Shandong Provincial H ospital,Jinan 250021,China;
2. Department of Cardiac Surgery,Qilu Hospital,Shandong University,Jinan 250012,China;
3. Academy of Military Medical Sciences,Beijing 100036,China) |
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| Abstract: | The results of current study show that the optimum sterilization of explants is 7-8 min with 0.1% HgCl 2.The concentration of NAA,6 BA affects the formation of callus,shoot induction and rooting of plantlets.A increase in 6 BA concentration resulted in increased callus formation.The BA/NAA ratio determined the shoot induction.Half strength MS medium proved best for the rooting plantlets and the addition of 6 BA inhibited it.The best media for different cultural stages are:(1)callus:MS+6 BA 1mg/L+NAA 0.1mg/L+charcoal 3%;(2)shoot induction:MS+ 6 BA 0.5mg/L+NAA 0.5mg/L; (3)rooting:1/2 MS+IBA 0.2mg/L. |
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| Keywords: | Anigozanthos( Kangaroo paws) Tissue culture |
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