黄瓜ZYMV-CH抗性遗传与连锁分子标记研究

 小西葫芦黄化花叶病毒中国株系( ZYMV-CH) 是危害我国黄瓜的主要病毒之一。本试验以抗病的‘秋棚’和感病的‘欧洲8号’杂交获得的115份重组自交系(R IL) 为材料, 进行了黄瓜抗ZYMV-CH遗传规律和连锁分子研究。结果表明: 病情指数在黄瓜R IL群体呈双峰分布, 表明其对ZYMV的抗性是受主基因控制的性状, 但也存在微效基因的修饰作用。采用集团分离分析法(BSA) 和AFLP技术, 获得与ZYMV-CH抗性基因连锁的两条特异性片段 E-ACG/M-CAG-182和E-ACG/M-CAG-180) , 遗传连锁距离分别为5 cM 和11 cM。将E-ACG/M-CAG-180 特异片段转化成共显性的SCAR 标记SCAR3-109, 与ZYMV-CH的抗性基因遗传连锁距离为11 cM。该标记可以作为黄瓜抗ZYMV辅助选择的分子标记。

The Resistance Genetic Analysis and Tightly Linked Molecular Markers for ZYMV-CH in Cucumber

The Chinese strain of zucchini yellow mosaic virus (ZYMV-CH) is a major disease in cucumbers. A RIL population of a cross between‘European 8’and‘Qiupeng’were used for virus resistance evaluations. The frequency distribution in cucumber RIL population for disease index involving resistance to the ZYMV-CH showed two peaks, which indicated that the resistance to this diseases was controlled by main gene, but some minor gene also had moderate effect. AFLP and BSA were adopted to identify DNA markerslinked to the resistance-related gene in the RIL6 population. Two markers, E-ACG/M-CAG-182 and E-ACG/M-CAG-180 were linked to it, and the genetic distanceswere 5 cM and 11 cM, respectively. The E-ACG/M-CAG-180 marker had transferred into a co-dominante SCAR marker ( SCAR3-109) , and the genetic distances was 11 cM. The result showed that this scarmarker could be useful in marker-assisted selection in ZYMV cucumber resistance beeding.