西瓜花叶病毒新疆鄯善分离物的纯化及不同甜瓜品种的抗病性鉴定

从新疆哈密瓜(Cucumis melo L.)主产区之一的鄯善县采集表现病毒病症状的哈密瓜嫩叶,经昆诺藜连续3次单斑分离纯化后,采用RT-PCR扩增、克隆和测序分析,将该分离物鉴定为西瓜花叶病毒(Watermelon mosaic virus,WMV)。利用该分离物,采用汁液摩擦接种法对12个常见的甜瓜商业品种和13个国际通用的甜瓜白粉病生理小种鉴别寄主进行室内抗病性鉴定,结果表明,所有供试品种中没有免疫和高抗品种,表现抗病、中抗、感病和高感的品种分别有1、11、10和3个,其中表现抗病的为Iran H。

Purification of a Watermelon mosaic virus Isolate from Shanshan County and Evaluation for the Virus Resistance of Various Melon Cultivars

As one of the most important economic crops in Xinjiang，Hami melon（Cucumis melo L.）is of high reputation over the world. However，virus diseases are currently a serious threat for sustainable production of Hami melon because of the lack of effective antivirus chemical and appropriate virus-control method. To develop virus-resistant varieties，young leaves of Hami melon with virus-infected symptoms were collected from Shanshan county，which is one of the major Hami melon growing locations in Xinjiang. This specimen was biologically cloned by three local lesion passages in Chenopodium quinoa. Such biologically cloned virus is hereafter called an isolate，which was further identified by RT-PCR amplification，molecular cloning and sequencing. The results showed that the purified isolate was Watermelon mosaic virus（WMV），which was then inoculated to some common commercial melon varieties and 13 international universal hosts for identification of melon powdery mildew physiological races by mechanical rubbing，the laboratory evaluation of virus resistance indicated that there was no immune and high resistance varieties in the present study. The number of resistance，moderate resistance，sensitivity，and high sensitivity varieties were 1，11，10，and 3，respectively，out of these，cultivar Iran H showed resistance to WMV isolate.