| 莴苣‘红帆’品种下胚轴愈伤组织诱导与植株再生 |
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| 引用本文： | 高辉,苟晓松,邓运涛,李旭锋.莴苣‘红帆’品种下胚轴愈伤组织诱导与植株再生[J].园艺学报,2002,29(5):486-489. |
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| 作者姓名： | 高辉 苟晓松 邓运涛 李旭锋 |
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| 作者单位： | (四川大学生命科学学院, 成都610064) |
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| 摘 要： | 莴苣下胚轴愈伤组织诱导时, MS+ 6-BA 0. 5 mg/ L+ 2, 4-D 1 mg/ L+ NAA 0. 1 mg/ L 效果最好,出愈率达94%; 愈伤组织分化为芽时, MS+ 6-BA 1. 0 mg / L+ NAA 0. 05 mg/ L 分化频率最高, 达100% ; 再生苗在1/ 2MS+ NAA 0. 5 mg/ L 中诱导生根成为完整植株。
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| 关 键 词： | 莴苣 下胚轴 愈伤组织 植株再生 |
| 文章编号： | 0513-353X(2002)05-0486-02 |
| 修稿时间： | 2001年11月26 |
In Vitro Plant Regeneration from Callus Induced from Hypocotyl of Lettuce (Lactuca sativa) |
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| Gao Hui,Gou Xiaosong,Deng Yuntao,and Li Xufeng.In Vitro Plant Regeneration from Callus Induced from Hypocotyl of Lettuce (Lactuca sativa)[J].Acta Horticulturae Sinica,2002,29(5):486-489. |
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| Authors: | Gao Hui Gou Xiaosong Deng Yuntao and Li Xufeng |
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| Institution: | Laboratory of Tumor Molecular Biology, Ningbo No.2 Hospital, Ningbo 315010, China |
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| Abstract: | Taking MS、1/ 2MS as basic media, adopting 6-BA、NAA、and 2, 4-D of different concentration composition to conduct t issue culture and plant regeneration from hypocotyl of lettuce, the result showed that medium of MS+ 6-BA 0. 5 mg/L+ 2, 4-D 1mg/L+ NAA 0. 1mg/ L , was the best to induce callus from hypocotyl, the rate of callus is 94%; and the differentiat ion medium, MS+ 6-BA 1. 0mg/ L + NAA 0. 05mg/ L, was the best to induce buds from callus and had a 100% plant regenerat ion rate; and it was for rooting media, 1/ 2MS+ NAA 0. 5 mg/ L, to induce root ing from seedlings. |
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| Keywords: | Lettuce(Lactuca sativa) Hypocotyl Callus tissue Plant regeneration |
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