| 金钗石斛花芽cDNA表达文库的构建及鉴定 |
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| 引用本文： | 庄军平,黄胜琴,潘舒群,叶庆生.金钗石斛花芽cDNA表达文库的构建及鉴定[J].园艺学报,2006,33(4):895-897. |
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| 作者姓名： | 庄军平 黄胜琴 潘舒群 叶庆生 |
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| 作者单位： | (华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广东广州510631) |
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| 基金项目： | 广东省自然科学基金;广东省高新技术成果(孵化)项目;广东省重大科技专项基金 |
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| 摘 要： | cDNA文库是分离、筛选基因的重要平台。利用SMARTTM cDNA library construction技术, 成功构建了金钗石斛花芽cDNA表达文库。结果表明该文库的克隆数为3.02 ×105 pfu, 插入片段的大小在0.5～2.5 kb范围, 插入片段的重组率接近100% , 说明所构建的cDNA文库质量较好。该文库的构建为克隆与金钗石斛花芽分化相关功能基因奠定了基础。
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| 关 键 词： | 金钗石斛 花芽 cDNA表达文库 |
| 文章编号： | 0513-353X（2006）04-0895-03 |
| 收稿时间： | 2005-09-14 |
| 修稿时间： | 2005-09-142005-12-01 |
Construction and Identification of a cDNA Expression Library from Dendrobium nobile |
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| Zhuang Junping,Huang Shengqin,Pan Shuqun,Ye Qingsheng.Construction and Identification of a cDNA Expression Library from Dendrobium nobile[J].Acta Horticulturae Sinica,2006,33(4):895-897. |
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| Authors: | Zhuang Junping Huang Shengqin Pan Shuqun Ye Qingsheng |
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| Institution: | (College of Life Science, South China Normal University, Guangdong Key Laboratory of Biotechnology for Plant Development,Guangzhou, Guangdong 510631, China) |
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| Abstract: | A cDNA expression library from the flower bud of Dendrobium nobile was constructed into pcDNA3.0 eukaryotical expression plasmid used SMARTTM cDNA library construction protocol. The result showed that the cDNA expression library contained 3.02 ×105 pfu, the size of the inserts ranges from 0.5 kbto 2.5 kb, and the percentage of positive clones was almost 100%. All of the above mentioned have answered for the general requirements of a cDNA library. This provides a base for cloning of important genes related toflower bud differentiation in Dendrobium nobile. |
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| Keywords: | Dendrobium nobile Flower bud cDNA expression library |
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