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莲种质花粉形态特征研究
引用本文:李佐,赵凯歌,赵玫,陈龙清.莲种质花粉形态特征研究[J].园艺学报,2015,42(1):75-85.
作者姓名:李佐  赵凯歌  赵玫  陈龙清
作者单位:1华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉 430070;2广东省农业科学院环境园艺研究所,广州 510640
基金项目:国家自然科学基金项目(31272197)
摘    要:利用扫描电镜对不同来源的31份莲种质的花粉形态进行了观察。研究结果表明:花粉外部形态均呈近球形,赤道面观均为椭圆形,极面观大部分呈三裂圆形,少量种质有近圆形。所有莲种质除都具3条萌发沟外,在5个种质中还首次发现具有散沟的花粉(六沟花粉)。不同种质间花粉形态特征具有差异,尤其是萌发器官和外壁纹饰细部特征的差别,体现了不同莲种质的遗传多样性和遗传分化,可为种质间亲缘关系及系统发育研究提供有价值的依据。

关 键 词:  花粉形态  种质资源  遗传多样性  扫描电镜

Studies on Pollen Morphology of Nelumbo Germplasm Resources
LI Zuo,ZHAO Kai-Ge,ZHAO Mei,CHEN Long-Qing.Studies on Pollen Morphology of Nelumbo Germplasm Resources[J].Acta Horticulturae Sinica,2015,42(1):75-85.
Authors:LI Zuo  ZHAO Kai-Ge  ZHAO Mei  CHEN Long-Qing
Institution:1Key Laboratory of Horticultural Plant Biology(Ministry of Education),College of Horticulture & Foresry Sciences,Huazhong Agricultural University,Wuhan 430070,China;2Environmental Horticulture Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China
Abstract:Pollens gains from 31 Nelumbo germplasm was examined using scanning electron microscopy(SEM). The results showed that all pollen grains investigated are sub-spheroidal in shape and elliptical in equatorial view. Pollen are predominately 3-lobed-rounder type in polar view,and the others are sub-orbicular. The pollen of all the accessions exclusively are 3-colpate type,and five accessions are additional 6-pantocolpate type. The obvious differences of pollen morphology of those 31 Nelumbo germplasm,especially in the characters of colpus and lirae demonstrated the genetic diversity and divergence,which provided valuable tools to analyze the phylogenetic relationship among the Nelumbo accessions.
Keywords:lotus  Nelumbo  pollen morphology  germplasm resource  genetic diversity  scanning electron microscopy (SEM)
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