葡萄PP2C家族基因的鉴定与表达分析

参考拟南芥(Arabidopsis thaliana)的PP2C基因注册序列,从葡萄(Vitis vinifera)全基因组中鉴定得到PP2C家族基因27个,分为10个亚族A、C~I、K、L。编码氨基酸181~1 084个,理论等电点4.46~8.98。亚细胞定位分析表明,葡萄PP2C基因家族主要在细胞质、叶绿体和细胞核中表达。二级结构主要以α–螺旋和不规则卷曲为主。葡萄PP2C基因芯片表达谱分析发现,该家族成员在葡萄不同组织中响应不同的逆境胁迫。qRT-PCR分析表明,葡萄试管苗在100和50 mmol·L~(-1) ABA、10% NaCl和10%PEG单独处理6 h后VvPP2C02的诱导表达量最高,分别是对照的11倍、8倍、14倍和13倍。

Genome-Wide Identification and Expression Analysis of the PP2C Gene Family in Vitis vinifera

In this study，27 members of the VvPP2C gene family were identified in the whole genome of grapes（Vitis vinifera L.），with reference to Arabidopsis thaliana PP2C gene registration sequence. The VvPP2C genes expression was analyzed by bioinformatics，microarray expression and stress analysis. The results showed that the 27 members of VvPP2C gene family could be divided into 10 subpopulations of A，C–I，K，L. The analysis of physicochemical properties showed that the number of amino acids in the VvPP2C gene family was between 181–1 084，and the theoretical pI distribution was between 4.46–8.98. The VvPP2C gene family was mainly expressed in the cytoplasm，chloroplast and nucleus through the subcellular localization analysis. The VvPP2C gene family was mainly composed of α-helix and irregular coil in secondary structure analysis. The microarray analysis showed that VvPP2C gene members of the family responded to different stress in different tissues of the grape. The inducible expression of grape plantlets was the highest in 100，50 mmol ? L-1ABA，10% NaCl and 10% PEG，at 11，8，14 and 13 times in qRT-PCR analysis，respectively.