| 农杆菌介导GNA基因转化菜薹 |
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| 引用本文： | 张扬勇,李汉霞,叶志彪,卢永恩,陆芽春.农杆菌介导GNA基因转化菜薹[J].园艺学报,2003,30(4):473-475. |
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| 作者姓名： | 张扬勇 李汉霞 叶志彪 卢永恩 陆芽春 |
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| 作者单位： | (华中农业大学作物遗传改良国家重点实验室，武汉430070) |
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| 摘 要： | 以菜薹带柄子叶为外植体，采用农杆菌介导法将特异启动子PRS,一l与雪花莲凝集素(GNA)基因构建的嵌合基因转化菜薹，获得26株Km抗性植株，经过PCR检测和PCR-Southem杂交分析证明其为转基因植株。
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| 关 键 词： | 菜薹 遗传转化 雪花莲凝集素基因(GNA) |
| 文章编号： | 0513-353X(2003)04-0473-03 |
| 修稿时间： | 2002年8月28日 |
Genetic Transformation of Flowering Stalk with Snowdrop Lectin Gene(GNA) |
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| Zhang Yangyong,Li Hanxia,Ye Zhibiao,Lu Yong''''en,and Lu Yachun.Genetic Transformation of Flowering Stalk with Snowdrop Lectin Gene(GNA)[J].Acta Horticulturae Sinica,2003,30(4):473-475. |
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| Authors: | Zhang Yangyong Li Hanxia Ye Zhibiao Lu Yong'en and Lu Yachun |
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| Institution: | (National Key Laboratory ofCrop Genetw Improvement，Huazhong Agricultural University， Wuhan 430070，China) |
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| Abstract: | Cotyledons with petiole of Flowering stalk were transformed with the vector carrying a snowdrop lectin gene( Galanthus nivalid Agglutinin, GNA ).Twenty six transformants were obtained from cotyledons of Flowering Stalk.They were confirmed to be transformants by the PCR and PCR Southern blot analysis. |
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| Keywords: | Flowering stalk Genetic transformation Snowdrop lectin gene( GNA ) |
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