| 南瓜矮生基因Bu的比较定位 |
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| 引用本文： | 王深浩,李海真,张忠华,贺俊,贾长才,张帆,黄三文.南瓜矮生基因Bu的比较定位[J].园艺学报,2011,38(1):95-100. |
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| 作者姓名： | 王深浩 李海真 张忠华 贺俊 贾长才 张帆 黄三文 |
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| 作者单位： | 1. 中国农业科学院蔬菜花卉研究所,农业部园艺作物遗传改良重点实验室,中荷联合园艺作物基因组技术实验室,北京100081
2. 北京市农林科学院蔬菜研究中心,北京,100097 |
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| 基金项目： | 农业部'948'项目，国家自然科学基金，'十一五'国家科技支撑计划项目 |
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| 摘 要： | 以中国南瓜矮生突变体为供体亲本，以印度蔓生南瓜为轮回亲本，构建了BC6F2分离群体。利用黄瓜基因组序列，将南瓜矮生基因Bu比较定位至黄瓜5号染色体，并开发了一个新的PCR标记IF3629，该标记与矮生基因Bu连锁遗传距离为1.0 cM。该标记不仅可以用于分子标记辅助选择育种，而且为Bu基因的克隆奠定了基础。
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| 关 键 词： | 南瓜 矮生基因 比较基因组学 分子标记 |
Comparative Mapping of the Dwarf Gene Bu from Tropical Pumpkin (Cucurbita moschata Duchesne) |
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| WANG Shen-hao,LI Hai-zhen,ZHANG Zhong-hua,HE Jun,JIA Chang-cai,ZHANG Fan,HUANG San-wen.Comparative Mapping of the Dwarf Gene Bu from Tropical Pumpkin (Cucurbita moschata Duchesne)[J].Acta Horticulturae Sinica,2011,38(1):95-100. |
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| Authors: | WANG Shen-hao LI Hai-zhen ZHANG Zhong-hua HE Jun JIA Chang-cai ZHANG Fan HUANG San-wen |
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| Institution: | WANG Shen-hao1,LI Hai-zhen2,ZHANG Zhong-hua1,HE Jun1,JIA Chang-cai2,ZHANG Fan2,and HUANG San-wen1,(1Key Laboratory of Horticultural Crops Genetic Improvement of Ministry of Agriculture,Sino-Dutch Joint Lab of Horticultural Genomics Technology,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China,2Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China) |
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| Abstract: | A segregating BC6F2 population was developed using a dwarf mutant of Cucurbita moschata Duch.as donor and the vine C.maxima Duch.'Mengri'as recurrent parent.Using the segregating population,the gene Bu was comparatively mapped on chromosome 5 of cucumber.An intron-flanking marker IF3629 was found to be tightly linked to the Bu gene,with a genetic distance of 1.0 cM.The marker IF3629 will not only be useful in markers-assisted selection for dwarf plant,but also provide a starting point toward positional isol... |
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| Keywords: | squash dwarf gene comparative genomics molecular marker |
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