| 利用大葱幼叶进行组织培养微繁的研究 |
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| 引用本文： | 张松,张启沛.利用大葱幼叶进行组织培养微繁的研究[J].园艺学报,1995,22(2):161-165. |
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| 作者姓名： | 张松 张启沛 |
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| 作者单位： | 山东农业大学园艺系 |
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| 摘 要： | 以大葱幼叶为外植体，成功地建立了大葱的无性繁殖系和高效的培养体系。在ＭＳ＋２，４－Ｄｌｍｇ／Ｌ＋ＫＴ０．５ｍｇ／Ｌ＋ＢＡｌｍｇ／Ｌ的培养基上，愈伤组织的诱导率为８９．９％，在ＭＳ＋２，４－Ｄ０．８８ｍｇ／Ｌ＋ＢＡ１．７３ｍｇ／Ｌ的培养基上达到最大芽诱导率１３．２１％，平均每块愈伤组织生芽５．１４个，在天激素的培养基上最易生根。２５０天内的繁殖系数可达３７８７倍。
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| 关 键 词： | 大葱 组织培养 愈伤组织 |
Studies on Propagation in Vitro Using Immature Leaf on Welsh Onion |
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| Zhang Song and Zhang Qipei.Studies on Propagation in Vitro Using Immature Leaf on Welsh Onion[J].Acta Horticulturae Sinica,1995,22(2):161-165. |
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| Authors: | Zhang Song and Zhang Qipei |
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| Abstract: | Vegetative propagation lines were bred by tissue culture in vitro using immature leaf segment.Calli grew rapidly and vigorously on MS medium containing 2,4-Dling/L and KT 0.sing/L and BA ling/L with the callus induction rate of 89. 9%.MS medium supplemented with 2,4-D 0.88mg/L and BA 1.73mg/L was suitable for adventitious shoot induction.The maximum shoot induction rate reached 13. 21%.Shoots rooted quite well on MS medium with no hormones.The propagation coefficient in 250days was 3787. |
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| Keywords: | Welsh onion:Tissue culture Callus |
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