黄瓜杂交二代抗黄瓜白粉病的蛋白质组学初步分析

通过分组分离法建立F₂代抗感池, 利用2-DE差异显示和质谱分析研究了抗病池和感病池的差异蛋白质组。抗感池经双向电泳检测到近500个蛋白点, 质谱分析鉴定出9种抗感池表达差异蛋白点,分别为抗病基因蛋白( SSP3110) , 14-3-3脑蛋白家族蛋白( SSP1411) , 粪卟啉原氧化酶Ⅲ ( SSP5513) , 碳酸酐酶( SSP5412 ) , α - 半乳糖苷酶( SSP5710 ) , 推定的蛋白( SSP6002 ) , 17.8 kDa 的热激蛋白( SSP0710) , 假想蛋白( SSP3111) , ( S) - 2 - 羟酸氧化酶( SSP4610) 。其中有3种蛋白的性质及生物学功能未知, 其余6种蛋白分别与光合作用、呼吸作用、抗病反应及信号转导等有密切关系。这些蛋白都可能是与抗性有关或与发育相关的蛋白网络中的一部分, 它们在黄瓜抗病反应中起着不同的作用。

Proteomic Analysis of F2 Generation of Cucumber Against the Cucumber Powdery Mildew Disease

Proteomic app roaches using two-dimensional gel electrohoresis (2-DE) were adop ted to identify proteins from cucumber leaves differentially exp ressed in F₂ generation of cucumber. Nearly 500 proteins were reproducibly detected, including 39 specific p roteins exp ressed in F2 sensitive pool samp les and 2-specific p roteins exp ressed in resistance pool samp les, 35 proteinswere two-fold upper limit and 46 proteins were two-fold lower limit. Analysiswith MALDI-TOF-MASS showed that nine important proteins associated with de2fensive reaction were identified in F₂ cucumber, a protein of disease resistance gene ( SSP3110) , 14-3-3 brain protein homolog ( SSP1411) , cop roporphyrinogen Ⅲ oxidase ( SSP5513) , carbonic anhydrase ( SSP5412) ,alpha2galactosid2ase2like p rotein ( SSP5710) , putative p rotein ( SSP6002) , 1718 kDa class small heat shock protein ( SSP0710) , hypothetical p rotein ( SSP3111) , ( S) -2-hydroxy-acid oxidase ( EC1.1.3.15) -cucur-bit ( SSP4610). All of those proteinswere probably parts of network of resistance or development-related proteins. This study gives new insights into cucumber powderymildew resistant response in cucumber leaves and demonstrates the power of the proteomic app roach in plant biology studies.