Relationship between radioresistance and microRNA in the same genetic background nasopharyngeal carcinoma cells

AIM:To discuss the relationship between the discrepancy of microRNA (miRNA) and radioresistance in nasopharyngeal carcinoma (NPC) cells CNE-2R and CNE-2 on the basis of validating their different radioresistance.METHODS:Following the exposure of X ray on the clones of CNE-2R and CNE-2 cells, the dose-survival curve and biological characteristics of CNE-2R and CNE-2 cells were determined by SigmaPlot software and the linear quadratic model of survival curve analysis.MicroRNAs were detected by μParafloTM microfluidic chip, hybridization images collected by a laser scanner (GenePix 4000B, Molecular Device) and the signals normalized by a LOWESS filter.The relationship between the discrepancy of NPC radioresistance and the expression of miRNA was predicted according to Targetscan3.1 database (http://www.targetscan.org) after analyzing the data.RESULTS:Compared to CNE-2 cells, 37 miRNAs were gain-of-function and 29 miRNAs were loss-of- function in CNE-2R cells among 719 detected miRNAs.12 miRNAs that detective value was more than 2 000 and 2 folds than the other were hsa-miR-200b, hsa-miR-224, hsa-miR-26b, hsa-miR-125a-5p, hsa-miR-205, hsa-let-7e, hsa-let-7g, hsa-miR-19b, hsa-miR-24, hsa-miR-103, hsa-miR-106b and hsa-miR-93.Data showed that the distinct discrepancy of miRNAs was related to radioresistance.CONCLUSION:The discrepancy of miRNAs is present in different radioresistant NPC cell lines and related to radioresistance.