冰核细菌对仁用杏胚珠超微结构的影响

 应用透射电镜对两个仁用杏品种‘白玉扁’和‘一窝蜂’接种冰核细菌并低温处理后的胚珠超微结构进行了观察, 发现冰核细菌对胚珠的超微结构有一定影响: (1) 使珠心细胞发生严重的质壁分离,细胞质中存在大量泡状结构, 线粒体的内部结构完全被破坏, 呈透明状, 而且有些珠心细胞中形成同心圆状的多膜内含物; (2) 使胚囊中卵细胞的细胞核外膜膨胀, 甚至核膜局部解体, 细胞质中细胞器减少。这些变化致使胚珠发育不正常, 影响受精而导致减产或绝收。接种冰核细菌并低温处理比单纯低温处理对胚珠超微结构的破坏程度重。

Effect of Ice Nucleation Active Bacteria on the Ultrastructure of Apricot Varieties Ovule

The ultrastructure of two kinds of apricot(Prunus armeniaca L.)ovule by ice nucleation active(INA)bacteria and low temperature was investigated with transmission electron microscopy.The results showed that the ultrastructure of ovule was influenced by the INA bacteria.(1)The plasmolysis phenomenon occurred in the nucellus cells,a great lot of vesicle structures existed in the cytoplasm.The structures of mitochondrion was entirely damaged and turned transparent.Furthermore there were multi-membrane inclusions with concentric circles in some nucellus cells.(2)The ectoblast nuclear membrane of ovum in embryo-sac was inflated.The nuclear membrane was disintegrated partly and number of organelles in cytoplasm decreased.Therefore,the ovule could not develop normally,which interfered the spermatiation and led to the reduction in apricot yield or even no output at all.In addition,compared with the treatment of only low temperature, the ovule ultrastructure by the treatment of both low temperature and INA bacteria inoculation were devastated more seriously.Therefore,INA bacteria was one important factor to aggravate frost injury to apricot flowers.