| 黄瓜枯萎病新型抑制蛋白L37的研究 |
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| 引用本文： | 郑爱萍,闫敏,李平,谭芙蓉,郑秀丽,李壮.黄瓜枯萎病新型抑制蛋白L37的研究[J].园艺学报,2005,32(6):1102-1104. |
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| 作者姓名： | 郑爱萍 闫敏 李平 谭芙蓉 郑秀丽 李壮 |
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| 作者单位： | (四川省农业生物技术工程研究中心, 成都611130) |
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| 基金项目： | 国家‘863’项目（2002AA212151）;四川‘十五’科技攻关项目 |
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| 摘 要： | 从土壤中分离到对黄瓜枯萎病病原菌有抑制作用的枯草芽孢杆菌(Bacillus subtilis) , 发酵产物经DEAE - 纤维素、Sephadex G-100柱层析, 分离到15 kD抑菌蛋白L37, SDS-PAGE显示纯度达到电泳纯, N - 末端氨基酸序列同源性检测结果提示可能是一种新型的抑菌蛋白。蛋白质抑菌结果表明, 对多种病原真菌具有抑制效果, 且对蛋白酶、温度和大部分有机物不敏感, 是稳定的抑菌蛋白。对病原菌菌丝抑制机理研究表明, 抑菌蛋白具有严重扭曲、断裂、阻止生长等抗生效果。应用发酵蛋白产物进行两年大田防效试验表明, 对黄瓜枯萎病防效显著, 最高达到90% , 具有显著提高产量的作用。
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| 关 键 词： | 黄瓜 黄瓜枯萎病 枯草芽孢杆菌 抑菌蛋白 防治 |
| 文章编号： | 0513-353X（2005）06-1102-03 |
| 收稿时间： | 2005-03-21 |
| 修稿时间： | 2005-03-212005-05-16 |
Research on New Antagonistic Protein L37 against Fusarium oxysporum |
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| Zheng Aiping,Yan Min,Li Ping,Tan Furong,Zheng Xiuli,Li Zhuang.Research on New Antagonistic Protein L37 against Fusarium oxysporum[J].Acta Horticulturae Sinica,2005,32(6):1102-1104. |
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| Authors: | Zheng Aiping Yan Min Li Ping Tan Furong Zheng Xiuli Li Zhuang |
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| Institution: | (Sichuan Provincial Center for Agri-Biotech Research, Chengdu 611130, China) |
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| Abstract: | Bacillus subtilis inhibiting cucumber wither pathogen was screened from soil. After fermenting, 15 kD antagonistic protein named L37 was got through DEAE-fibrin, Sephadex G-100 chomatography, which proved purified completely through SDS-PAGE. It was probably a new type of antagonistic protein against pathogen by the sequence on the amino N-terminal testing, which could make the normal hypha of pathogen distort, split and inhibit. The control effect could reach 90% against cucumber wither pathogen and improved the yield in field controlling test by fermenting products. |
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| Keywords: | Cucumber Cucumber wither Bacillus subtilis Antagonistic protein Controlling |
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