盐胁迫下丛枝菌根真菌对番茄吸水及水孔蛋白基因表达的调控

 采用盆栽试验研究不同浓度（0.5%和1.0%）NaCl胁迫下接种丛枝菌根真菌Glomus mosseae-2 对番茄吸水特性的影响，并用realtime-PCR技术分析了根系中5个水孔蛋白基因（LePIP1，LePIP2，LeTRAMP，LeAQP2，LeTIP）的表达。结果表明：盐胁迫下接种丛枝菌根真菌能促进植株生长和对水分的吸收，显著提高叶片相对含水量、叶片水势及根系水导；丛枝菌根真菌和盐胁迫共同调控了5个水孔蛋白基因的表达；与未接菌株相比，接种植株LeAQP2基因表达上调，其余4个基因表达下调。LeAQP2基因在接菌株根中的过量表达与盐胁迫下丛枝菌根真菌提高番茄根系水导有关。

Regulative Effect of Arbuscular Mycorrhizal Fungi on Water Absorption and Expression of Aquaporin Genes in Tomato Under Salt Stress

Effect of arbuscular mycorrhizal fungi on regulation of water absorption and expression of five aquaporin genes（LePIP1，LePIP2，LeTRAMP，LeAQP2，LeTIP）of root in arbuscular mycorrhizal tomato by realtime-PCR were studied under different NaCl stress（0.5%，1.0%）. Results showed that the growth of tomato was improved，as while relative water content，water potential and hydraulic conductance of root were increased by AMF inoculation under salt stress. Expression of five genes was induced by AMF and salinity jointly. Up-regulated expression of LeAQP2 gene and down-regulated of other four genes in root of AMF tomato were detected in contrast to non-AMF one. Accordingly，overexpression of LeAQP2 gene in root may be relation to enhanced hydraulic conductance induced by AMF.