湖北海棠二价融合表达载体MhT2AC的构建及转化烟草耐盐性分析

 利用口蹄疫病毒2A序列将湖北海棠MhTGA2转录因子和几丁质酶基因（Mhchitl）两个抗病相关基因融合在同一个开放阅读框下，构建二价融合植物双元表达载体，命名为MhT2AC。将该载体转化烟草，获得了8个转基因株系，MhTGA2基因和Mhchitl基因在烟草中共表达，并且转基因株系中NtSOD、NtAPX和NtPPO等抗逆基因的表达量均加强；转基因烟草T₁代植株抗盐性增强。

Construction of a Divalent Plant Binary Expression Vector MhT2AC from Malus hupehensis and Analysis of Salinity Tolerance in the Tobacco Transformants

The plant binary expression vector containing the divalent genes of MhTGA2 and Mhchit1 isolated from Malus hupehensis and fused in a single open reading frame(ORF)by Foot and mouth disease virus(FMDV)were constructed.The vector was then introduced into tobacco via Agrobacterium-mediated.The expression of NtSOD,NtAPX and NtPPO genes were detected and salinity tolerance was evaluated in the tobacco transformants.The results showed that we successfully constructed a plant binary expression vector with bivalent fusion and denoted it as MhT2AC.The MhTGA2 gene and the Mhchit1 gene were co-expression in the transformants.Comparing with the controls,the expression of NtSOD,NtAPX and NtPPO genes were enhanced in transgenic lines.Moreover,the salinity tolerance of the T1 transformants were significantly improved.