多花蔷薇假珠芽诱导、体细胞胚发生及植株高效再生

 以多花蔷薇‘无刺3号’成熟种子为外植体，探讨了假珠芽诱导、体细胞胚发生及植株高效再生的方法。结果表明，种子的子叶在添加2, 4-D 0.5～2 mg·L-1的1/2 MS培养基上暗培养30 d后所产生的愈伤组织，接种到添加TDZ 5～20 mg·L-1的1/2 MS培养基上光培养20 d产生初级假珠芽。假珠芽在添加TDZ 10 mg·L-1和GA3 0.1 mg·L-1的诱导培养基上暗培养20 d后,在含麦芽糖的无激素培养基上光培养产生少量次级假珠芽和胚性愈伤组织。假珠芽保存在诱导培养基上。胚性愈伤组织可发育成大量正常体细胞胚，继而再生正常植物。假珠芽可通过上述方法不断诱导体细胞胚和假珠芽，通过这种方法假珠芽已经保存了2年。

Pseudobulbils Induction,Somatic Embryogenesis and Shoot Regeneration in Rosa multiflora Thunb.

A protocol for the induction of somatic embryogenesis via pseudobulbils from mature seeds of Rosa multiflora 'inermis 3' was established. The results showed that the primary pseudobulbils were obtained by using a two-stage procedure where excised seed cotyledons were incubated 30 days on the 1/2 Murashige and Skoog (MS) medium supplemented with 2, 4-D at 0.5~2 mg·L^-1 under the dark conditions for calli induction, and subsequently transferred to a 1/2 MS medium supplemented with TDZ at 5~20 mg·L^-1 under the light conditions for 20 days for pseudobulbils induction. The pseudobulbils were incubated 20 days on the induction medium supplemented with TDZ at 10 mg·L^-1 under the dark conditions and then transferred to the hormone-free medium supplemented with maltose instead of sucrose under light conditions for the secondary pseudobulbils and embryogenic calli induction. The pseudobulbils were maintained on the induction medium. The embryogenic calli developed into normal somatic embryos then plantlets. By this way, the pseudobulbils have been maintained for two years by far.