| 外源Ca2+ 和ABA 对高温下黄瓜花药中Ca2 + 、ABA及蛋白质合成的影响 |
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| 引用本文： | 缪旻珉,朱月林,张玉华.外源Ca2+ 和ABA 对高温下黄瓜花药中Ca2 + 、ABA及蛋白质合成的影响[J].园艺学报,2005,32(1):111-114. |
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| 作者姓名： | 缪旻珉 朱月林 张玉华 |
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| 作者单位： | (扬州大学农学院园艺系, 扬州225009; 南京农业大学园艺学院, 南京210095) |
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| 基金项目： | 国家自然科学基金资助项目(30100123) |
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| 摘 要： | 外源Ca2 +能降低高温下黄瓜单核期花药的胞内Ca2 +水平并提高花粉萌发率, 而ABA的作用与此相反。外源Ca2 +和ABA处理能显著提高高温下黄瓜花药内源ABA的水平, 并产生新的蛋白质。以上结果表明, 高温下外源Ca2 +可影响黄瓜花药内ABA信号系统, 继而可能改变基因表达, 并获得耐热性;外源ABA亦能影响黄瓜花药内Ca2 +信号系统。
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| 关 键 词： | 黄瓜 高温 花药 钙 ABA 蛋白质双向电泳 |
| 文章编号： | 0513-353X(2005)01-0111-04 |
| 收稿时间： | 2004-05-08 |
Effect of Exogenous Ca2+ and ABA on Ca2+ Distribution, ABA Contents and Proteins Synthesis in Cucumber Anthers under Heat Stress |
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| Miao Minmin,Zhu Yuelin,Zhang Yuhua.Effect of Exogenous Ca2+ and ABA on Ca2+ Distribution, ABA Contents and Proteins Synthesis in Cucumber Anthers under Heat Stress[J].Acta Horticulturae Sinica,2005,32(1):111-114. |
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| Authors: | Miao Minmin Zhu Yuelin Zhang Yuhua |
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| Institution: | (Department of Horticulture, College of Agriculture, Yangzhou University, Yangzhou 225009, China; College of Horticulture, Nanjing Agriculture University, Nanjing 210095, China) |
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| Abstract: | Exogenous Ca2+ reduce the intercellular Ca2 + of cucumber anthers and increase pollen germination percentage at high temperature, while the exogenous ABA have a reverse effect. Endogenous ABA contents in two line anthers ascend significantly linked with new protein formation after exogenous Ca2 + and ABA treatment under 45℃. The results indicated that Ca2 + and ABA signals can interact each other in cucumber uninucleate pollen stage anthers under high stress. |
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| Keywords: | Cucumber High temperature Anther Ca 2 ABA 2D PAGE |
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