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温度对GF43李离体保存中老化作用的影响
引用本文:马瑞娟,俞明亮,许建兰,宋卫平,洪法水,万志刚.温度对GF43李离体保存中老化作用的影响[J].园艺学报,2004,31(5):660-662.
作者姓名:马瑞娟  俞明亮  许建兰  宋卫平  洪法水  万志刚
作者单位:( 江苏省农业科学院园艺研究所, 南京210014 ; 苏州大学生命科学学院, 苏州215006)
基金项目:江苏省自然科学基金项目 (BK2 0 0 2 5 0 1)
摘    要: 研究了不同温度对GF43李( Prunus domestica L. ) 试管苗生长及老化过程的影响。结果表明在16 ℃保存条件下GF43试管苗的营养生长缓慢, 8 ℃保存GF43试管苗的营养生长极缓慢, SOD、CAT 和POD 等保护酶系统的活性维持较高水平, 体内O2 自由基、丙二醛(MDA) 含量较低, 细胞膜结构相对稳定, 从而延缓了试管苗的衰老, 延长了两次继代之间的时间, 对试管苗的保存十分有利。

关 键 词:  温度  离体保存  衰老
文章编号:0513-353X(2004)05-0660-03
收稿时间:2003-10-17
修稿时间:2004-2-2

Effects of Temperature on Senescence of GF43 (Prunus domestica L.) Plantlet in Vitro
Ma Ruijuan,Yu Mingliang,Xu Jianlan,Song Weiping,Hong Fashui,and Wan Zhigang.Effects of Temperature on Senescence of GF43 (Prunus domestica L.) Plantlet in Vitro[J].Acta Horticulturae Sinica,2004,31(5):660-662.
Authors:Ma Ruijuan  Yu Mingliang  Xu Jianlan  Song Weiping  Hong Fashui  and Wan Zhigang
Institution:( Institute of Horticulture , Jiangsu Academy of Agricultural Sciences,Nanjing 210014 , China ; College of L if e Sciences,Suzhou University , Suzhou 215006 , China)
Abstract:The growth and senescence of GF43 ( Prunus domestica L. ) cultured at different temperatures in vit ro were studied. The results showed that the growth rate of GF43 plantlet in vitro was slow at 16 ℃, the activities of superoxide dismutase (SOD) , catalase (CAT) and peroxidase ( POD) were incresed , the activated oxygen scarenging system was improved. Consequently this alleviated the harmful effect of O·2 and reduced the membrane lipid peroxidation in shoot , prolonged the life-spane of GF43 plantlet in vitro.
Keywords:Prunus domestica L    Temperature  Plantlet in vitro  Senescence
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