| 猕猴桃成熟果实中脂氧合酶基因的克隆 |
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| 引用本文： | 陈昆松 Ross.,GS.猕猴桃成熟果实中脂氧合酶基因的克隆[J].园艺学报,1998,25(3):230-235. |
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| 作者姓名： | 陈昆松 Ross. GS |
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| 作者单位： | 浙江农业大学园艺系(陈昆松)，新西兰国家研究院园艺与食品研究所(张上隆) |
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| 基金项目： | 国家自然科学基金,浙江省自然科学基金,农业部“九五”重点项目资助 |
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| 摘 要： | 采用ＰＣＲ扩增方法，从成熟猕猴桃果实组织中克隆到一个８２４ｂｐ的脂氧合酶（ＬＯＸ）基因片段（ｐＫＬＣ１），该片段含有２７５个氨基酸组成的开放阅读框架，它与拟南芥菜、黄瓜、豌豆、土豆、水稻、大豆、烟草和番茄的核苷酸同源性为５９．０％～７４．６％，氨基酸同源性为６３．１％～７６．３％。Ｓｏｕｔｈｅｒｎ杂交表明，ｐＫＬＣ１片段为一低拷贝数基因家族所编码。
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| 关 键 词： | 猕猴桃 果实 脂氧合酶 基因 |
Isolation of A Lipoxygenase cDNA from Ripening Actinidia chinensis Fruit |
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| Chen Kunsong ,Zhang Shanglong ,and Gavin S.Ross.Isolation of A Lipoxygenase cDNA from Ripening Actinidia chinensis Fruit[J].Acta Horticulturae Sinica,1998,25(3):230-235. |
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| Authors: | Chen Kunsong Zhang Shanglong and Gavin SRoss |
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| Institution: | Chen Kunsong 1,Zhang Shanglong 1,and Gavin S.Ross 2 |
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| Abstract: | An 824bp cDNA fragment(pKLC1)encoding part of the lipoxygenase(LOX)protein was cloned by PCR from ripening Actinidia chinensis fruit.pKLC1 has an open reading frame containing 275 amino acids. Comparisions between pKLC1 sequence and others from Arabidopsis ,cucumber,pea,potato,rice,soybean,tobacco and tomato indicated nucleic acid homology between 59% and 75%,and amino acid identity between 63% and 76%. Southern analysis confirmed that pKLC1 is encoded by a low copy number gene in A.chinensis. |
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| Keywords: | Actinidia chinensis Fruit Lipoxygenase Gene |
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