果树组培苗瓶外滤纸桥生根技术研究

 对珠美海棠、樱桃colt 砧木、弗吉尼亚草莓组培苗进行瓶外滤纸桥生根试验,结果表明: 珠美海棠、弗吉尼亚草莓瓶外生根率较瓶内高20. 1 %和4. 1 %; 樱桃colt 砧木与瓶内生根基本相同; 瓶外生根时间均缩短至3～10 d ; 移栽成活率较瓶内分别提高16. 1 %、21. 4 %和14. 9 % , 达到93 %以上; 根的活跃面积分别提高6. 9 %、11. 54 %和7. 5 %; 珠美海棠上表皮、下表皮、栅栏组织厚度及气孔密度分别较瓶内生根苗高45. 8 %、50. 3 %、47. 6 %和2. 5 %; 该方法可降低成本75 %以上。

Research on Outer Bottle Rooting Technique of Fruit Seedling in Vitro with Filterpaper Bridge

The rooting technique of fruit tissue culture system with filter paper bridge were studied in this experiment.The results indicated that the rooting rates of Zhumei crabapple and strawberry with filter paper bridge were 20% and 4.1% more than those of their controls respectively;the rooting rate of cherry stock was same as that of its control,but the rooting time was decreased to 3-10 days;the transplanting survival rates of these three kinds of fruit tree plantlets all increased up to 93%,the survival rates of Zhumei crabapple,strawberry and cherry stock were increased by 16.1%,21.4% and 14.9% respectively,compared with their controls;the root vigor areas of Zhumei crabapple,Strawberry and cherry stock were increased by 6.9%,11.54% and 7.5%,compared with their controls;the thickness of epicuticle,hypodermis and pajisade tissue and the leaf stoma density of Zhumei crabapple were increased by 45.8%,50.3%,47.6% and 2.5% respectively,compared with its control.This method is simple and convenient.The cost of tissue culture could be cut down 75% than traditional rooting methods.