| 苹果离体叶片培养直接体细胞胚胎发生研究 |
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| 引用本文： | 达克东,张松.苹果离体叶片培养直接体细胞胚胎发生研究[J].园艺学报,1996,23(3):241-245. |
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| 作者姓名： | 达克东 张松 |
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| 作者单位： | 山东农业大学遗传所 园艺系 农学系 |
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| 摘 要： | 在ＭＳ＋ＢＡ１０ｍｇ／Ｌ＋ＮＡＡ５０ｍｇ／Ｌ＋２，４＿Ｄ０＼＾５ｍｇ／Ｌ＋蔗糖２０ｇ／Ｌ的固体培养基上，苹果品种嗄拉试管苗叶片经７天暗培养产生胚性细胞。将具有胚性细胞的叶片转入ＭＳ＋ＢＡ１０ｍｇ／Ｌ＋蔗糖２０ｇ／Ｌ的培养基上暗培养４０天，６５％的叶片直接发生体细胞胚，体细胞胚在同种培养基上萌发，长成小植株。
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| 关 键 词： | 苹果：离体叶片培养 体细胞胚 小植株 |
Direct Somatic Embryogenesis from in Vitro Leaves of Apple |
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| Da Kedong ,\ Zhang Song,\ Li Yazhi ,\ and Qi Zengjun.Direct Somatic Embryogenesis from in Vitro Leaves of Apple[J].Acta Horticulturae Sinica,1996,23(3):241-245. |
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| Authors: | Da Kedong \ Zhang Song \ Li Yazhi \ and Qi Zengjun |
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| Institution: | Da Kedong 1,\ Zhang Song2,\ Li Yazhi 1,\ and Qi Zengjun 3 |
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| Abstract: | Embryogenic Cells Were induced from in vitro leaves of apple cultivar `Gala' after 7 days in the dark on MS medium supplemented with 1 0 g/L BA, 5 0mg/L NAA, 0 5mg/L 2,4_D and sucrose 20 0g/L. Sixty five percent of the leaves regenerated somatic embryos directly when transferred to MS medium with 1 0mg/L BA and sucrose 20 0g/L in the dark for 40 days. The regenerated embryos were going to germinate and develop into shoots on the same medium. |
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| Keywords: | Apple Leaf culture in Vitro Somatic Embryo shoot |
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