核桃硬壳发育期内果皮木质素与相关酶活性的#br# 变化

 以‘赞美’和‘珍珠香’核桃（Juglans regia L.）果实为试材，采用间苯三酚–HCl 法观察 了内果皮木质化过程，测定了其发育过程中木质素、纤维素和酚类物质含量以及相关酶活性的变化。结 果表明：两个品种核桃硬壳木质化始于6 月初，6 月中旬初步成型，7 月下旬完成。从硬壳木质化至坚果 采收，木质素、纤维素和酚类物质含量均呈逐渐升高趋势，但酚类物质含量在采前略有下降，POD 和PAL 活性均呈下降趋势，PPO 活性整体变化不大。相关分析表明，核桃硬壳分化期木质素含量与纤维素含量 极显著正相关，酚类物质含量与木质素含量极显著正相关，与纤维素含量显著正相关；POD 活性与木质 素、纤维素、酚类物质含量极显著负相关，与PAL 活性极显著正相关。

Changes of Lignin Content and Its Related Enzyme Activities in Endocarp#br# During Walnut Shell Development Period

Lignification process was observed by the method of Phloroglucinol-HCl and content of lignin，cellulose，polyphenol and the activity of related enzymes were measured during development with the materials of walnut of‘Zanmei’and‘Zhenzhuxiang’. The results showed that lignification occurred at beginning of June，already initial took shape in mid-June and completed in late July. From shell lignification to harvest，the content of lignin，cellulose and polyphenol was increased，while polyphenol content slightly decreased before harvest. The activities of both POD and PAL were decreased and PPO varied little. The correlation analysis showed that lignin content was significant positive correlated with the content of cellulose. Polyphenol content was significant positive correlated with the content of lignin. Polyphenol content was positively correlated with cellulose. The activity of POD was significant negative correlated with content of lignin，cellulose and polyphenol，but positively correlated with activity of PAL.