菠菜性别基因X/Y连锁标记的筛选及应用

以菠菜杂交一代品种‘冬绿1号’中的雌株与雄株杂交构建的F2分离群体为试验材料,采用SRAP-BSA法筛选与性别基因X/Y连锁的分子标记。从256对SRAP引物组合中筛选到了3个与性别基因X/Y紧密连锁SRAP标记:SRAP4.3、SRAP5.7和SRAP9.5。将SRAP5.7和SRAP9.5分别转化为SCAR标记(S5.7、S9.5),将SRAP4.3转化为d CAPs标记(D4.3)。SCAR标记S5.7、S9.5与Y基因共分离,d CAPs标记D4.3与X/Y基因紧密连锁,遗传距离为0.3 c M。利用S5.7、S9.5和D4.3在其它7个菠菜群体中进行雌雄性别鉴定,分子标记鉴定结果与表型鉴定结果一致,因此S5.7、S9.5和D4.3可以用于分子标记辅助选择育种。

Development and Application of Molecular Markers Linked with Sex Gene X/Y in Spinach

The F2 segregation population constructed by hybridization among female and male individuals of‘Winter Green 1’was our experimental material. We obtained the molecular markers linked with the sex gene X/Y using SRAP-BSA technique. Three SRAP markers（SRAP4.3，SRAP5.7，SRAP9.5）closely linked with the sex gene X/Y were gained from 256 SRAP primer combinations. SRAP5.7 and SRAP9.5 were successfully converted into SCAR markers（S5.7，S9.5）. SRAP4.3 was smoothly transformed into dCAPs marker（D4.3）. S5.7 and S9.5 marker were co-segregated with Y gene. D4.3 marker was closely linked to X/Y gene，and the genetic distance is 0.3 cM. The sex in other 7 spinach populations was identified by the marker S5.7，S9.5 and D4.3. The results of molecular identification were consistent with the phenotype，so these three molecular markers could be used for marker-assisted selection breeding（MAS）.