| 大白菜—甘蓝型油菜单体异附加系的获得与鉴定 |
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| 引用本文： | 李丹丹,李雪红,彭莹,方小雪,万正杰,徐跃进.大白菜—甘蓝型油菜单体异附加系的获得与鉴定[J].园艺学报,2014,41(7):1355-1360. |
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| 作者姓名： | 李丹丹 李雪红 彭莹 方小雪 万正杰 徐跃进 |
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| 作者单位： | 华中农业大学园艺林学学院，国家蔬菜改良中心华中分中心，园艺植物生物学教育部重点实验室，武汉 430070 |
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| 基金项目： | 国家科技支撑计划项目（2013BAD20B06） |
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| 摘 要： | 以甘蓝型油菜作为母本，大白菜作为轮回父本，杂交获得BC1 和BC2 群体。通过染色体核
型分析法从BC2 群体中筛选出大白菜—甘蓝型油菜C 基因组6 号单体异附加系，利用甘蓝型油菜C 连锁
群特异SSR 标记鉴定，确定该单体异附加系中的外源染色体与甘蓝型油菜C6 连锁群相对应。
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| 关 键 词： | 大白菜 甘蓝型油菜 单体异附加系 核型分析 SSR |
Generation and Identification of Chinese Cabbage–Cabbage Type Rape Monomer Alien Addition Line |
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| LI Dan-Dan,LI Xue-Hong,PENG Ying,FANG Xiao-Xue,WAN Zheng-Jie,XU Yue-Jin.Generation and Identification of Chinese Cabbage–Cabbage Type Rape Monomer Alien Addition Line[J].Acta Horticulturae Sinica,2014,41(7):1355-1360. |
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| Authors: | LI Dan-Dan LI Xue-Hong PENG Ying FANG Xiao-Xue WAN Zheng-Jie XU Yue-Jin |
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| Institution: | College of Horticulture and Forestry，Huazhong Agricultural University；National Center for Vegetable Improvement，
Centeral China；Key Laboratory of Horticulture Plant Biology，Ministry of Education，Wuhan 430070，China |
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| Abstract: | In this experiment，BC1 and BC2 populations were derived from a distant hybridization between cabbage type rape（Brassica napus）and Chinese cabbage（B. compestris ssp. pekinensis），using the latter as recurrent parent. A Chinese cabbage with cabbage type rape monosomic alien addition line was identified from the BC2 population through karyotype analysis. SSR marker analysis showed that the monosomic alien addition line was introduced from C-chromosome 6 of cabbage type rape. |
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| Keywords: | Chinese cabbage cabbage type rape monosomic alien additional line karyotype analysis SSR |
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