| 羽衣甘蓝花粉SCR13-b基因的分离及其功能分析 |
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| 引用本文： | 蓝兴国,杨佳,李玉花.羽衣甘蓝花粉SCR13-b基因的分离及其功能分析[J].园艺学报,2009,36(4):539-544. |
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| 作者姓名： | 蓝兴国 杨佳 李玉花 |
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| 作者单位： | (东北林业大学生命科学学院, 哈尔滨150040) |
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| 摘 要： | 以羽衣甘蓝(Brassica oleracea var. acephala) S13-bS13-b自交不亲和系为试验材料, 分离了控制花粉自交不亲和基因———S13-b单倍型富含半胱氨酸基因(SCR13-b) , GenBank收录号为EF577028。SCR13-b cDNA序列含有237 bp的开放读码框(ORF) , 编码一个含78个氨基酸的蛋白。SCR13-b gDNA含有一个758 bp的内含子, 内含子5′供体位点和3′受体位点边界序列符合GU2AG规则; DNA blot分析表明SCR13-b在基因组中只有单一拷贝; 授粉生物活性分析试验显示原核表达SCR132b融合蛋白能够启动SI反应, 抑制杂交亲和花粉的萌发和生长。
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| 关 键 词： | 羽衣甘蓝 自交不亲和 原核表达 |
| 收稿时间： | 2008-10-27 |
| 修稿时间： | 2009-3-19 |
Isolation and Functional Analysis of a S13-b-IOCUS Cysteine-rich Gene of Orna-mental Kale (Brassica oleracea var. acephala) |
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| LAN Xing-guo,YANG Jia,LI Yu-hua.Isolation and Functional Analysis of a S13-b-IOCUS Cysteine-rich Gene of Orna-mental Kale (Brassica oleracea var. acephala)[J].Acta Horticulturae Sinica,2009,36(4):539-544. |
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| Authors: | LAN Xing-guo YANG Jia LI Yu-hua |
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| Institution: | (College of L ife Sciences, N ortheast Forestry University, Harbin 150040, China) |
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| Abstract: | The S13-b-locus cysteine-rich gene (SCR13-b) was isolated from pollen of ornamental kale (Brassica oleracea var. acephala) S132b homozygotes, GenBank accession number is EF577028. The SCR13-b cDNA sequence had an ORF of 237 bp, encoding a 782aa p rotein. The genomic SCR13-b sequence contained a 758 bp intron with boundary sequences of GT at 5′site and AG at 3′site, which comp lied with the typical‘GT-AG rule’in plants. Genomic DNA blot analysis showed that SCR13-b was a single copy gene in S13-b S13-b lines. Pollination bioassay showed that prokaryotic expressed recombinant SCR13-b can induce SI response and inhibit cross pollen germination and growth. |
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| Keywords: | Brassica oleracea var acephala Brassica oleracea var acephala self-incompatibility prokaryotic expression |
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