| 丽格海棠叶片培养胚状体发生和植株再生 |
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| 引用本文： | 达克东,张松,高东升,米瑞芙,周志文,束怀瑞.丽格海棠叶片培养胚状体发生和植株再生[J].园艺学报,2001,28(2):180-185. |
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| 作者姓名： | 达克东 张松 高东升 米瑞芙 周志文 束怀瑞 |
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| 作者单位： | (山东农业大学园艺系, 泰安271018) |
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| 摘 要： | 利用丽格海棠盆栽植株叶片培养, 成功地诱导出胚状体并获得再生植株。具体步骤如下: Ⅰ. 在MS + BA 1 mg/L + NAA 4 mg/L + 2 ,42D 1 mg/L 培养基上预诱导10 d ; Ⅱ. 在MS + BA 1 mg/L 培养基上胚性细胞发生胚状体; Ⅲ. 在MS + IBA 0. 5 mg/L 培养基上小植株生根。组织切片观察表明, 胚状体起源于叶片上表皮细胞。
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| 关 键 词： | 秋海棠 组织培养 胚状体 |
| 文章编号： | 0513-353X(2001)02-0180-02 |
Somatic Embryogenesis from Leaves of Begonia×Elatior |
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| Da Kedong,Zhang Song,Gao Dongsheng,Mi Ruifu,Zhou Zhiwen,Shu Huairui.Somatic Embryogenesis from Leaves of Begonia×Elatior[J].Acta Horticulturae Sinica,2001,28(2):180-185. |
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| Authors: | Da Kedong Zhang Song Gao Dongsheng Mi Ruifu Zhou Zhiwen Shu Huairui |
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| Institution: | 1.College of Life Science and Technology, ;2.Institute for Tissue Transplantation & Immunology, Jinan University, Guangzhou 510632, China |
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| Abstract: | Somatic embryo formation was induced from leaves of Begonia×Elatior by a three-step-method：1.Embryonic cells were prelimin ary induced on MS+BA 1 mg/L+NAA 4 mg/L+2，4-D 1 mg/L medium 10 days；2.Somatic embryo formation was observed on MS+BA 1 mg/L medium；3.Roots were induced on MS+IBA 0.5 mg/L mediu m.Histological study showed that the embryoid come from upper epidermal cell of the leaf. |
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| Keywords: | Begonia ×Elatior Begonia ×Elatior Tissue culture Somatic embryo |
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