| 利用CRISPR/Ca9技术靶向编辑芥蓝BoaZDS |
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| 引用本文： | 郑爱红,张 芬,江 敏,袁 巧,江雷雨,陈 清,汤浩茹,孙 勃.利用CRISPR/Ca9技术靶向编辑芥蓝BoaZDS[J].园艺学报,2019,46(1):57-64. |
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| 作者姓名： | 郑爱红 张 芬 江 敏 袁 巧 江雷雨 陈 清 汤浩茹 孙 勃 |
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| 作者单位： | （四川农业大学园艺学院，成都 611130） |
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| 摘 要： | 以芥蓝(Brassicaoleraceavar.alboglabra)为材料,以ζ–胡萝卜素脱氢酶(ζ-Carotene desaturase,ZDS)基因为目标基因,建立其CRISPR/Cas9基因组编辑体系。在BoaZDS的编码区近5′端选择靶位点,构建了CRISPR/Cas9表达载体,通过农杆菌介导的遗传转化方法获得了19个芥蓝转基因阳性植株,Sanger测序分析发现其中13株成功突变,CRISPR/Cas9载体在芥蓝上的突变效率为68.42%,且所有突变植株均表现出明显的白化表型。
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| 关 键 词： | 芥蓝 CRISPR/Cas9 基因组编辑 ζ–胡萝卜素脱氢酶（ZDS） |
Targeted Editing of BoaZDS by CRISPR/Ca9 Technology in Chinese Kale |
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| ZHENG Aihong,ZHANG Fen,JIANG Min,YUAN Qiao,JIANG Leiyu,CHEN Qing,TANG Haoru,SUN Bo.Targeted Editing of BoaZDS by CRISPR/Ca9 Technology in Chinese Kale[J].Acta Horticulturae Sinica,2019,46(1):57-64. |
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| Authors: | ZHENG Aihong ZHANG Fen JIANG Min YUAN Qiao JIANG Leiyu CHEN Qing TANG Haoru SUN Bo |
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| Institution: | （College of Horticulture，Sichuan Agricultural University，Chengdu 611130，China） |
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| Abstract: | The ζ-carotene desaturase（ZDS）gene was selected as the target gene to establish the CRISPR/Cas9 genome editing system in Chinese kale. The target site，near the 5′ end of the coding region of the BoaZDS，was selected，and the CRISPR/Cas9 expression vector was constructed. As a result，a total of 19 transgenic plants were obtained through Agrobacterium-mediated genetic transformation. Sanger sequencing analysis revealed that 13 of them were mutated. The mutation efficiency was 68.42%，and all mutants showed a clear albino phenotype. |
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| Keywords: | Chinese kale CRISPR/Cas9 genome editing ζ-carotene desaturase（ZDS） |
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