柑橘胼胝质合成酶基因家族的表达分析

胼胝质由胼胝质合成酶合成,胼胝质沉积是植物对入侵病原的防御反应。为了探索胼胝质合成酶(Callose synthase,CalS)基因在柑橘中对黄龙病的应答情况,从甜橙基因组中筛选并利用生物信息学分析了柑橘胼胝质合成酶(CsCalS)家族的12个基因。这些基因编码的氨基酸序列与拟南芥中的胼胝质合成酶具有较高的同源性。荧光实时定量PCR结果显示,CsCalS5和CsCalS12在柑橘健康叶片中的表达量高于其他胼胝质合成酶基因。比较黄龙病感病材料和健康对照材料,发现CsCalS5、CsCalS7和CsCalS8在感病的‘砂糖橘’和‘强德勒’柚中均上调表达,可能参与柑橘对黄龙病病原的应答反应。

Expression Analysis of Callose Synthase Gene Family in Citrus

Callose deposition is a common plant defense response to invasive pathogens and involved in plant innate immunity. In order to explore the expression of callose synthase（CalS）in citrus and the response to Huanglongbing（HLB），12 members of callose synthase genes（CsCalSs）were screened from the sweet orange（Citrus sinensis）genome and analyzed with bioinformatic tools. The coding amino acid sequences of these CsCalS genes showed high homology with callose synthase gene in Arabidopsis thaliana. The results produced by real time PCR revealed that the expression of CsCalS5 and CsCalS12 in citrus leaves were higher than that of other callose synthase genes. On the other hand，CsCalS5，CsCalS7 and CsCalS8 were all up-regulated in HLB-affected‘Shatangju’（C. reticulata）and‘Chandler’pummelo（C. grandis）comparing with the healthy control，indicated they may be involved in the response of citrus to the HLB pathogen.