弱光和短日照下‘幻想’矮牵牛花芽代谢组学研究

分析了强光长日照(对照,光强11.5μmol·m~(-2)·s~(-1),16 h)、弱光长日照(3.6μmol·m~(-2)·s~(-1),16 h)、强光短日照(11.5μmol·m~(-2)·s~(-1),8 h)处理下‘幻想’矮牵牛花芽的形态发育及代谢情况。对花芽的观测统计表明,弱光和短日照分别导致了正常花芽和总花芽数量极显著减少和败育花芽显著增加。结合谱库检索技术与质谱图解析及保留指数对物质成分进行了鉴定,采用多元统计分析和峰面积归一化法进行了相对定量计算。结果共找到111个峰,鉴定出42种物质;弱光和短日照处理与对照的花芽代谢差异明显,主要表现为多种氨基酸含量增多,糖类代谢物减少;其中弱光照比短日照对花芽代谢的影响更大。初步推测弱光或短日照处理下败育花产生的原因之一是光胁迫导致了花芽内各种蛋白质和糖类消耗的增加。

Study on the Flower Bud Metabolomics of Petunia hybrida‘Fantasy’Grown Under Weak Light or Short Day Conditions

The flower bud number and metabolism of Petunia hybrida‘Fantasy’under the condition of control（light strength 11.5 μmol ? m-2 ? s-1，day length 16 h），weak light（3.6 μmol ? m-2 ? s-1，16 h）and short day（11.5 μmol ? m-2 ? s-1，8 h）were observed and recorded. Observation on flower buds showed that the number of normal and total buds decreased dramatically while aborted buds increased significantly under either the weak light or short day stress. Metabolites were identified by mass spectra retrieval and spectra resolution combined with retention index. Then，we went on multivariate statistical analysis and determined semi-quantitatively by peak area normalization method. The results were as follows：we found out 111 peaks and 42 of them were identified. There was significant difference in metabolism of flower bud of Petunia hybrida‘Fantasy’under the condition of control，weak light and short day. Compared with control，the metabolic shifts was shown as the increasing of various amino acids and the decreasing of various sugars. And weak light had larger influence on metabolism of the flower bud than that of short day. Depletion of proteins and sugars was reckoned to be one of the reasons that lead to aborted flower bud under the weak light or short day stress.