侵染广东辣椒的辣椒脉斑驳病毒的分子特征

辣椒脉斑驳病毒（Chilli veinal mottle virus，ChiVMV）是引起广东辣椒病毒病的主要病原之一，为了明确侵染为害广东辣椒的ChiVMV分子特征，采用分段RT-PCR和RACE扩增方法克隆了ChiVMV广东分离物（ChiVMV-GD）的基因组全序列，结果表明，除poly（A）尾外，ChiVMV-GD基因组大小为9 721 nt，编码一个大小350.44 kD多聚蛋白（位于167 ~ 9 436 nt），5′–非编码区（5′-UTR）和3′–非编码区（3′-UTR）分别含有166和285 nt，5′–末端结合病毒基因组连接蛋白（VPg），3′–末端含有poly（A）尾。序列同源性分析结果表明：ChiVMV-GD与ChiVMV其他分离物的基因组序列同源率为79.1% ~ 96.9%，其中与来自中国海南分离物（登录号：GQ981316.1）的同源率最高（96.9%）。系统进化分析结果显示，ChiVMV-GD与海南分离物聚集在一个小分支，说明与海南分离物亲缘关系最近。

Molecular Characterization of Chilli veinal mottle virus Infecting Pepper in Guangdong Province

Chilli veinal mottle virus（ChiVMV）is one of main pathogen causing pepper virus disease in Guangdong Province，China. To ascertain the molecular characterization of ChiVMV isolate Guangdong（ChiVMV-GD），the whole genome sequence of ChiVMV-GD was cloned by RT-PCR and RACE amplification. In addition to the poly（A）tail，the length of ChiVMV-GD genome was 9 721 nt and encoded a 350.44 kD polyprotein（coordinate 167–9 436 nt）. The 5′-untranslated region（5′-UTR）and 3'-untranslated region（3′-UTR）contained 166 and 285 nt，respectively. The 5′-end contained a viral genome-linked protein（VPg）. The 3′-end contained a poly（A）tail. The whole genome sequence of ChiVMV-GD shared 79.1% to 96.9% nucleotide sequence identities with other isolates of ChiVMV deposited in the GenBank database，with the highest nucleotide sequence identities to the isolate Hainan（GenBank accession number：GQ981316.1）at 96.9%. Phylogenetic analysis of ChiVMV-GD and the other 11 isolates of ChiVMV indicated that it clustered with the Hainan isolate to form a unique clade. This result showed that ChiVMV-GD had the closest relationship with ChiVMV Hainan isolate. This paper is the first report the molecular characterization of ChiVMV infecting pepper in Guangdong Province，China.