大白菜叶色突变体的HRM鉴定及其叶绿素荧光参数分析

将大白菜经甲基磺酸乙酯（EMS）诱变种子获得的42株叶色突变体按照生殖时期叶片颜色和叶绿素含量分为9种类型：深绿色、灰绿色、绿色、浅绿色、白绿色、白浅绿色、黄绿色、黄浅绿色、黄色；利用高分辨率熔解曲线（high resolution melting，HRM）技术对叶绿素荧光基因HCF164突变进行了筛选并结合叶绿素荧光参数测定，获得了1株黄绿色高光合效率突变体A29，1株黄绿色光合结构损伤突变体A35和1株浅绿色光合电子传递受阻突变体A21；对另外7个叶色相关基因的突变进行了HRM鉴定，表明叶绿素相关基因ATRCCR、CLH2、PORA突变可能是造成18个突变体叶色变化的主要原因，黄叶特异基因家族YLS突变与叶色变化也有关系。

HRM Identification and Chlorophyll Fluorescence Characteristics on Leaf Color Mutants in Chinese Cabbage

Forty-two leaf color mutants of Chinese cabbage obtained through EMS seeds mutagenesis were used as materials in this study. According to leaf color and leaf chlorophyll content at generative growth mutations were suggested to be divided into 9 types：Dark green，gray-green，green，light green，white-green，light white-green，yellow-green，light yellow-green and yellow. By detecting the nucleotide variation of the gene HCF164 related to chlorophyll fluorescence using HRM technology and by measuring chlorophyll fluorescence characteristics，we identified one yellow-green leaf color mutant A29 with high photosynthesis efficiency，one yellow-green leaf color mutant A35 with photosynthetic structure damages，one light green mutant A21 with photosynthetic electron transport obstruction. Through identifying other 7 leaf-color-related genes by HRM，mutation of chlorophyll-related genes ATRCCR，CLH2 and PORA could be the main reason resulted in 18 leaf color mutants，mutation of yellow-leaf- specific genes was also affected the variation of leaf color.