基于蛋白质组学研究光质对番茄果实挥发性物质的影响机理

以‘Micro-Tom’番茄(Solanum lycopersicum L.)为试材,种子发芽后30d移入人工气候室,分别进行红/蓝光组合(1:1、3:1、5:1、7:1)处理,以白光处理为对照。在番茄果实绿熟期、转色期、成熟期取样,测定果实挥发性物质含量及蛋白组数据。结果表明,在转色期和成熟期,对番茄风味有积极作用的己醛、反式–2–己烯醛、β–紫罗兰酮、牻牛儿丙酮、6–甲基–5–庚烯–2–酮、2–苯乙醇、愈伤木酚的含量在红/蓝光3︰1组合处理中最高,而对风味有消极作用的水杨酸甲酯的含量较低,并且在成熟期为0。进一步分析了红/蓝光3︰1组合处理与对照相比的差异蛋白,在成熟过程中番茄果实共鉴定到12个与挥发性物质产生有关的差异蛋白,其中8个上调表达,4个下调表达。通过对12个差异蛋白进行mRNA水平的验证发现,只有转色期的苯丙酮酸互变异构酶(MIF)在蛋白质和mRNA水平表达不一致,其他11个蛋白在两个水平表达均一致。

Volatile Compounds in Tomato Fruits Under Different Light Qualities Revealed by Proteomic Analyses

We used tomato（Solanumly copersicum L.）‘Micro-Tom’as the test material，after 30 days of seed germination. The seedlings were transferred to the artificial climate chamber and exposed to the following light conditions：red/blue combined light（1︰1），red/blue combined light（3︰1），red/blue combined light（5︰1），red/blue combined light（7︰1），and white light as the control. Fruits were harvested at the mature green，breaker，and ripe stages，then we determined the fruit volatile compounds content and proteomic. The results showed that in breaker and ripe stages of tomato fruits，hexanal，trans-2-hexenal，β-ionone，geranylacetone，6-methyl-5- hepten-2-one，2-phenylethanolx，callus lignan，which have positive effects on tomato flavor were the highest in the red/blue combined light（3︰1）treatment. The content of methyl salicylate，which has a negative effect on tomato flavor was low，and no methyl salicylate was detected at the ripe stage. To further elucidate the effects of light quality on volatile compounds content of tomato fruit，total protein was extracted from the control and red/blue combined light（3︰1）. A subsequent proteomic analysis revealed that twelve proteins related to volatile compounds were differentially abundant between the control and red/blue combined light（3︰1）during ripening. Among the twelve differentially abundant proteins related to volatile compounds，eight were up-regulated and four were down-regulated. The differential abundances of the twelve proteins were validated by determining the expression levels of the corresponding genes by qRT-PCR. The expression levels of eleven of the genes were consistent with the abundances of the corresponding proteins. There was a lack of consistency in the gene expression levels and protein abundances for phenylpyruvate tautomerase（MIF）.