Effect of FGFR1 expression knock-down on biological characteristics of infantile hemangioma endothelial cells |
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Authors: | XU Nan XU Jie LI Han QIAN Li-juan LI Zhong-tang |
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Institution: | 1. Department of Pediatrics, Wuxi Branch Hospital of Southeast University, Wuxi 214011, China;
2. Department of Pediatrics, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, China |
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Abstract: | AIM: To study the effect of fibroblast growth factor receptor 1 (FGFR1) expression knock-down on the viability, apoptosis, invasion and migration of infantile hemangioma endothelial cells (HemECs). METHODS: FGFR1 was down-regulated by FGFR1 small interfering RNA (si-FGFR1) transfection. The viability of the cells was measured by CCK-8 assay. The apoptotic rate was analyzed by flow cytometry and the invasion and migration abilities were determined by Transwell assay. The protein levels of phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), and phosphorylated AKT (p-AKT) were examined by Western blot. RESULTS: Transfection of si-FGFR1 into HemECs had significant effects on inhibiting cell viability (P<0.05), promoting apoptosis (P<0.05), and decreasing cell invasion and migration abilities (P<0.05). The results of Western blot showed that knockdown of FGFR1 gene expression in the cells reduced the protein levels of PI3K and p-AKT (P<0.05), and had no significant effect on AKT protein level. CONCLUSION: Knock-down of FGFR1 expression changes the biological characteristics of endothelial cells in infantile hemangiomas by regulating PI3K/AKT signaling pathway. |
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Keywords: | Hemangioma Fibroblast growth factor receptor 1 PI3K/AKT signaling pathway Apoptosis Invasion |
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