Effects of HuR on cell viability,migration and invasion of gastric cancer cell line MGC-803 |
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| Authors: | CAO Xiao-mei CAO Nan-jing WANG Fu-hua SUN Rui-fang LI Feng GUO Su-tang |
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| Institution: | 1. Department of Biochemistry and Molecular Biology, Basic Medical College, Shanxi Medical University, Taiyuan 030001, China;
2. Department of Molecular Biology, Shanxi Provincial Cancer Institute, Taiyuan 030013, China;
3. Sample Library, Shanxi Provincial Cancer Hospital, Taiyuan 030013, China |
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| Abstract: | AIM:To investigate the effects of HuR on cell function of gastric cancer cell line MGC-803. METHODS:The mRNA expression level of HuR was detected by RT-qPCR in the tumor samples of 80 gastric cancer patients diagnosed clinically. HuR gene knock-down was achieved by transfection of si-HuR into the MGC-803 cells. The invasion, migration and viability of MGC-803 cells were measured by the scratch wound hearing, Transwell and CCK-8 assays, respectively. RESULTS:High mRNA expression of HuR was observed in 67 cases (84%) of gastric cancer tissues as compared with their control samples. Furthermore, knock-down of HuR expression effectively inhibited the invasion, migration and viability of the MGC-803 cells (P<0.05), indicating that HuR play an important role in gastric cancer as an oncogene. CONCLUSION:Abnormal expression of HuR is correlated with the progression of gastric cancer. Knock-down of HuR expression inhibits the invasion, migration and viability of MGC-803 cells. |
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| Keywords: | HuR gene Gastric cancer Cell viability Cell migration Cell invasion |
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