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利用ISSR分析澳洲坚果的亲缘关系
引用本文:郭凌飞,邹明宏,杜丽清,曾辉,陆超忠,刘晓静.利用ISSR分析澳洲坚果的亲缘关系[J].园艺学报,2011,38(9):1741-1745.
作者姓名:郭凌飞  邹明宏  杜丽清  曾辉  陆超忠  刘晓静
作者单位:1. 中国热带农业科学院南亚热带作物研究所,广东湛江524091/广西壮族自治区亚热带作物研究所,南宁530001
2. 中国热带农业科学院南亚热带作物研究所,广东湛江,524091
3. 广西壮族自治区甘蔗研究所,南宁,530007
基金项目:农业科技成果转化资金项目(04EFN216900382); 云南省科技计划项目(2006YX16); 国家基础条件平台项目子项目(2005DKA21005-27)
摘    要: 从100条ISSR引物中筛选出19条重复性好,扩增条带清晰的引物对17份澳洲坚果种质进行亲缘关系分析。19条引物共扩增出369个位点,多态性位点249个(67.5%)。利用UPGMA法得到的聚类的结果表明17份种质分为4个类群,所有夏威夷选育出的品种在最大的一个类群中归于两个亚类,而在澳大利亚选育出的品种则分别聚为独立的类群,因此推测夏威夷选育出的品种很可能起源于两个遗传多样性不同的群体;而澳大利亚选育的品种其遗传背景和选育史与夏威夷的品种不同。

关 键 词:澳洲坚果  亲缘关系  ISSR
收稿时间:2011-3-21
修稿时间:2011-8-16

Analysis on Genetic Relations of Macadamia by ISSR Markers
GUO Ling-fei,ZOU Ming-hong,DU Li-qing,ZENG Hui,LU Chao-zhong, LIU Xiao-jing.Analysis on Genetic Relations of Macadamia by ISSR Markers[J].Acta Horticulturae Sinica,2011,38(9):1741-1745.
Authors:GUO Ling-fei    ZOU Ming-hong  DU Li-qing  ZENG Hui  LU Chao-zhong  LIU Xiao-jing
Institution:1. Department of Pathology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of TCM, Guangzhou 510120, China; 2. Department of Pharmacology,School of Medicine, Jinan University, Guangzhou 510632, China; 3. Department of physiology, School of Medicine, Jinan University, Guangzhou 510632, China; 4. Department of Pathology,Guangdong Medical College, Zhanjiang 524023, China.
Abstract:Nineteen primers were used to analysis the genetic relation of 17 macadamia which screened from 100 primers.A total of 369 bands were amplified,of which 249 bands were polymorphic bands(the percentage of polymorphic band,PPB = 67.5%).Examination of multivariate relationships among accessions using a cluster analysis resulted in the identification of four clusters.All Hawaiian cultivars were included in two sub-clusters within the largest cluster.The results suggesting that the Hawaiian cultivars must have o...
Keywords:macadamia  genetic relation  ISSR  
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