转沙冬青锌指蛋白基因AmZFPG 烟草非生物胁迫抗性分析

 分析了沙冬青（Ammopiptanthus mongolicus）锌指蛋白基因AmZFPG 在非生物胁迫下的表达 特性，结果显示AmZFPG 受低温、干旱、高盐胁迫诱导表达，表明该蛋白参与多种胁迫相关的信号转导 和应答反应。为进一步探索AmZFPG 的功能，构建了真核表达载体AmZFPG-pCAMBIA2300，将该基因 转入烟草（Nicotiana tabacum L.），对转基因烟草T1 代进行非生物胁迫分析，结果显示，转基因烟草的耐 寒性、耐旱性、耐盐性均获得了提高。

Overexpression of a Zinc-finger Protein Gene AmZFPG from Ammopiptanthus mongolicus Confers Tolerance to Cold ， Drought and Salt Stress in Transgenic Tobacco

The characterization of a novel gene designated AmZFPG from Ammopiptanthus mongolicus was described here. AmZFPG encodes a zinc-finger protein that is induced after different types of stresses，namely cold，desiccation，and salt. Overexpression of the gene in transgenic tobacco conferred tolerance to cold，dehydration，and salt stress at the seed-germination/seedling stage as reflected by the percentage of germination，the fresh weight of seedlings，and their growth pattern. Thus，AmZFPG seems to be an important determinant of stress response.